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本文采用Bradford法的改良法,对感染伯氏鼠疟(P.b,ANKA株)及用酮替芬、青蒿素、放线菌酮、氯喹、威灵仙5种抗疟药治疗后小鼠血清ASP进行定量测定。结果显示:感染疟疾小鼠血清ASP含量显著高于正常小鼠,其浓度与疟原虫感染率成正比关系;用以上5种抗疟药治疗后,小鼠血清ASP含量显著降低,但有差异,其浓度与抗疟药对疟原虫的抑制率成反比关系。以上结果提示,血清ASP定量测定,可作为疟疾病情监护和判定抗疟药疗效的一项指标,亦是抗疟药筛选的一种较好方法。
In this paper, Bradford method of improved method, the infection of mouse berberine (Pb, ANKA strain) and with ketotifen, artemisinin, cycloheximide, chloroquine, Clematis 5 kinds of anti-malarial drugs after treatment of mouse serum ASP for quantitative determination. The results showed that the serum ASP level of malaria-infected mice was significantly higher than that of normal mice, and its concentration was directly proportional to the infection rate of malaria parasite. After treatment with the above five kinds of antimalarial drugs, the ASP level of serum in mice decreased significantly, Its concentration is inversely proportional to the rate of inhibition of Plasmodium by antimalarial drugs. The above results suggest that serum ASP quantitative determination can be used as an indicator of malaria condition monitoring and judgments on the efficacy of antimalarial drugs and is also a good method for screening antimalarial drugs.