论文部分内容阅读
目的揭示水通道蛋白1.3.8在维吾尔族妇女宫颈癌发生、浸润过程中的表达及其与宫颈癌预后的关系。方法在慢性宫颈炎和宫颈癌组织中采用实时荧光定量RT-PCR方法检测AQP1,AQP3和AQP8的mRNA表达,使用免疫组织化学技术研究在慢性宫颈炎、宫颈上皮内瘤变Ⅲ级和浸润癌组织中的定位表达。结果 AQP1、AQP3和AQP8mRNA在慢性宫颈炎组织、早期宫颈癌及晚期宫颈癌均有表达,表达量之间差异无统计学意义(P>0.05)。AQP1、AQP3和AQP8蛋白在慢性宫颈炎、宫颈上皮内瘤样病变Ⅲ级(CINⅢ级)和浸润癌组织中均表达,表达于细胞膜,阳性率的差异有统计学意义(P<0.05)。宫颈癌的临床分期、肿瘤直径和淋巴结转移可作为判断患者预后的独立因素。AQP1、3、8可能通过局部肿瘤体积生长影响患者预后。结论 AQP1、3、8在宫颈癌发生、侵袭过程中可能发挥重要的作用。
Objective To reveal the expression of aquaporin 1.3.8 in cervical carcinogenesis and infiltration of Uighur women and its relationship with the prognosis of cervical cancer. Methods The mRNA expressions of AQP1, AQP3 and AQP8 were detected by real-time fluorescence quantitative RT-PCR in chronic cervicitis and cervical cancer. Immunohistochemistry was used to detect the expression of AQP1, AQP3 and AQP8 in chronic cervicitis, cervical intraepithelial neoplasia grade Ⅲ and invasive carcinoma In the positioning of expression. Results AQP1, AQP3 and AQP8 mRNA were all expressed in chronic cervicitis, early cervical cancer and advanced cervical cancer. There was no significant difference in the expression of AQP1, AQP3 and AQP8 mRNA (P> 0.05). The expression of AQP1, AQP3 and AQP8 protein in chronic cervicitis, cervical intraepithelial neoplasia grade Ⅲ (CIN Ⅲ grade) and invasive cancer were all expressed in the cell membrane, the difference was statistically significant (P <0.05). The clinical stage of cervical cancer, tumor diameter and lymph node metastasis can be used as independent factors to judge the prognosis of patients. AQP1,3,8 may influence the prognosis of patients by local tumor volume growth. Conclusion AQP1, 3 and 8 may play an important role in the development and invasion of cervical cancer.