一、水稻胚胎培养:(一)胚培养:根据培养目的可分为成熟胚培养和幼胚培养。前者对条件和技术的要求比较简单,后者比较复杂。1.成熟胚培养:将米粒消毒后接种在培养基上,当胚萌发长到约1厘米时,在芽鞘与胚乳的交界处逐渐膨大,形成愈伤组织,15天左右达到高峰,随愈伤组织的生长,胚芽开始枯萎。愈伤组织诱导强度以 First, rice embryo culture: (a) embryo culture: According to the purpose of culture can be divided into mature embryo culture and immature embryo culture. The former conditions and technical requirements are relatively simple, the latter is more complicated. 1. Mature embryo culture: After inoculation, the rice seeds are inoculated on the medium. When the germination of the embryo grows to about 1 centimeter, it gradually expands at the junction of the bud sheath and the endosperm to form the callus and reaches the peak around 15 days. Wound tissue growth, germ began to wilt. Callus induction intensity to