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长链非编码RNA(long noncoding RNA,lncRNA)的保守性表现在一级结构、空间结构、转录位置、剪接模式及组织分布等方面,是目前lncRNA研究的热点和难点。不断深入的lncRNA保守性研究可应用于参考基因组相对匮乏的非模式生物lncRNA的筛选过程,并且极大地提升了非模式生物lncRNA数据库建立的完整性和准确性。借助针对开放阅读框长度、密码子分布与出现频率、功能性结构域等保守信息开发而来的lncRNA筛选工具或流程如CPC、PLAR(pipeline for lncRNA annotation from RNA-seq data)等,已成为目前非模式生物lncRNA的筛选及其参考数据库构建的新策略。本文就lncRNA的保守性及其在非模式生物lncRNA筛选中的应用作一综述,并简要介绍了一种运用其保守性的筛选方法——PLAR。
The conservation of long noncoding RNA (lncRNA) in primary structure, spatial structure, transcriptional location, splicing patterns and tissue distribution are the hot and difficult issues in current lncRNA research. In-depth lncRNA conservation studies can be applied to the screening of non-model lncRNAs that are relatively deficient in the genome and greatly improve the integrity and accuracy of lncRNA database construction in non-model organisms. The lncRNA screening tools or procedures such as CPC, PLAR (Pipeline for lncRNA annotation from RNA-seq data), etc. have been developed with the aid of conservative information such as length of open reading frame, distribution and frequency of codons and frequency of functional domains, Screening of non - model lncRNA and a new strategy of constructing its reference database. This review summarizes the conservation of lncRNA and its application in lncRNA screening of non-model organisms, and briefly introduces a PLAR that uses its conservative screening method.