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目的研究四川汉族,西藏藏族和凉山彝族中HNA-3的分布频率,并比较产生抗-HNA-3a的理论风险。方法收集600例无偿献血员外周血标本,包括四川汉族献血者232例,西藏藏族献血者224例,凉山彝族献血者144例,采用PCR-SBT测序法对HNA-3进行分型。直接计数法计算基因型和等位基因频率。结果 HNA-3a和HNA-3b等位基因的频率分别在四川汉族人群为0.65和0.35,西藏藏族人群为0.66和0.34,凉山州彝族人群为0.60/0.40,3组之间没有统计学差异。基因型HNA-3a携带者(HNA-3a/a及HNA-3a/b)和HNA-3b/b分别在四川汉族人群为0.89和0.11,西藏藏族人群为0.88和0.12,凉山州彝族人群为0.81和0.19,汉族HNA-3b/b携带者显著低于彝族(P<0.05)。结论彝族产生抗-HNA-3a的可能性比汉族和藏族大,汉族和藏族产生抗-HNA-3a的可能性相当,该数据为不同地区产生抗-HNA-3a的可能性提供一定数据支持。
Objective To investigate the distribution of HNA-3 in Han, Tibetan and Liangshan Yi in Sichuan Province and to compare the theoretical risk of anti-HNA-3a. Methods Peripheral blood samples of 600 blood donors were collected, including 232 blood donors in Sichuan, 224 blood donors in Tibet and 144 blood donors in Liangshan. HNA-3 was genotyped by PCR-SBT. Direct counting method to calculate genotype and allele frequency. Results The frequencies of HNA-3a and HNA-3b alleles were 0.65 and 0.35 in Sichuan Han population, 0.66 and 0.34 in Tibetan Tibetans, 0.60 / 0.40 in Yi people in Liangshan Prefecture, respectively. There was no statistical difference between the three groups. The genotypes of HNA-3a carriers (HNA-3a / a and HNA-3a / b) and HNA-3b / b were 0.89 and 0.11 respectively in Sichuan Han population, 0.88 and 0.12 in Tibetan Tibetans, and 0.81 in Liangshan Prefecture And 0.19, Han HNA-3b / b carriers were significantly lower than the Yi (P <0.05). Conclusions The possibility of producing anti-HNA-3a in Yi population is comparable to that of Han, Tibetan, Han nationality and Tibetan people. This data may provide some data for the possibility of producing anti-HNA-3a in different areas.