论文部分内容阅读
目的:探讨银杏叶提取物(GBE)对肝肺综合征(HPS)大鼠血管扩张因子内皮素-1(ET-1)和降钙素基因相关肽(CGRP)的影响。方法:雄性成熟SD大鼠32只,随机平均分为4组:模型组、正常对照组、GBE低剂量组、GBE高剂量组,采用胆总管结扎术(CBDL)成功制备大鼠HPS模型,喂养3周后统一处死,用放射免疫分析法检测大鼠血浆和肝组织、肺组织匀浆中ET-l和CGRP的水平。结果:在大鼠HPS形成过程中,模型组、GBE低剂量组、GBE高剂量组血浆(血清)和肝、肺组织匀浆中ET-1、CGRP水平显著升高,经GBE治疗后显著下降,且GBE高剂量组更为显著。结论:GBE可降低HPS大鼠血浆(血清)和肝、肺组织匀浆中ET-1和CGRP水平,且高剂量比低剂量的治疗效果明显。
Objective: To investigate the effects of Ginkgo biloba extract (GBE) on the expression of endothelin-1 (ET-1) and calcitonin gene related peptide (CGRP) in rats with hepatopulmonary syndrome (HPS). Methods: Thirty-two male Sprague-Dawley rats were randomly divided into 4 groups: model group, normal control group, low-dose GBE group and high-dose GBE group. HPS model was successfully established by common bile duct ligation (CBDL) After 3 weeks, the rats were sacrificed and the levels of ET-1 and CGRP in the plasma and liver tissue and lung homogenates were determined by radioimmunoassay. Results: During the process of rat HPS formation, the levels of ET-1 and CGRP in plasma (serum), liver homogenate and lung homogenate of model group, GBE low dose group and GBE high dose group were significantly increased , And GBE high dose group is more significant. CONCLUSION: GBE can reduce the levels of ET-1 and CGRP in plasma (serum), liver and lung homogenates of HPS rats, and the effect of high dose and low dose of GBE is obvious.