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采用阳极氧化法制备新型高强度的TiO2纳米管薄膜,通过对纳米管底部进行HF气体腐蚀获得了两端通透的TiO2纳米管阵列薄膜.利用混合种植方法于两端通透的纳米管阵列表面种植了猪肾小管上皮细胞(LLC-PK1)和血管内皮细胞(ECV304),成功制备了具有生理功能的TiO2纳米管生物膜材料.运用血浆复钙化法对比研究了载玻片、纯金属钛片、未种植细胞TiO2纳米管和种植细胞TiO2纳米管的血液相容性,并采用自制的装置检测了该生物膜对钠钾离子的重吸收功能.结果表明,种植细胞的纳米管阵列薄膜的血液相容性要远远好于其他对照组,且该生物膜具有很好的重吸收功能,证实所制备的TiO2纳米管阵列生物膜具有良好的生理功能,是用于生物透析较为理想的候选材料.
A new type of high-strength TiO2 nanotube thin film was prepared by anodic oxidation method, and the TiO2 nanotube array film with both ends permeable was obtained by HF gas etching at the bottom of the nanotube. The hybrid nanotubes Porcine renal tubular epithelial cells (LLC-PK1) and vascular endothelial cells (ECV304) were planted and biofilm materials with biological function were successfully prepared.Compared with glass slides, pure titanium metal sheets , Uncoated cell TiO2 nanotubes and planting cell TiO2 nanotubes, and the self-made device was used to detect the reabsorption of sodium and potassium by the biofilm.The results showed that the blood of the nanotube array membrane The compatibility is much better than that of other control groups, and the biofilm has a good reabsorption function. It is confirmed that the prepared biomimetic TiO2 nanotube array has good physiological functions and is an ideal candidate material for bio-dialysis .