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目的:为了阐明不同的干旱条件及氮素水平对长春花叶片中4种生物碱(文多灵、长春质碱、长春碱、长春新碱)含量的影响,为提高长春花生物碱含量提供理论指导。方法:实验设置了CK(对照组即自然生长)、CN(对照+施氮素)、LK(轻度干旱)、LN(轻度干旱+施氮素)、HK(重度干旱)、HN(重度干旱+施氮素)6个处理组,探讨各处理组条件下长春花叶片全氮含量、碱性POD活性、TDC活性、4种生物碱含量的变化特性。结果:轻度干旱条件下POD,TDC的酶活性在处理的整个时期均提高,且胁迫的早期(0～21 d),有利于文多灵、长春质碱、长春碱、长春新碱的积累,后期(28～35 d)反而起抑制作用4种生物碱含量降低。施加外源氮素能有效提高各组长春花叶片中全氮含量、POD和TDC酶活性、文多灵和长春质碱的含量,干旱+施加氮素(LN,HN)处理下有利于长春碱和长春新碱的积累。结论:干旱胁迫及施加氮素对长春花叶片中POD,TDC的活性及4种生物碱的含量均能产生影响,轻度干旱条件下施加氮素(LN)对文多灵、长春质碱、长春碱、长春新碱含量的提高最为显著,是通过全氮含量及酶活性提高来调控的。
OBJECTIVE: To elucidate the effects of different drought conditions and nitrogen levels on the contents of four alkaloids (vinpocetine, vinblastine, vinblastine and vincristine) in vinca virescens leaves and to provide theoretical basis for increasing the content of alkaloids guide. Methods: CK (control, natural growth), CN (control + N application), LK (mild drought), LN (mild drought + N application), HK (severe drought), HN Drought and nitrogen application) were used to investigate the changes of total nitrogen content, alkaline POD activity, TDC activity and the content of four alkaloids in the leaves of each treatment group. Results: The activity of POD and TDC in mild drought condition increased during the whole period of treatment, and the early (0 ~ 21 d) of stress was conducive to the accumulation of vinpocetine, vinblastine, vinblastine and vincristine , Late (28 ~ 35 d) but the inhibition of the four alkaloids decreased. The application of exogenous nitrogen could effectively increase the content of total nitrogen, the activities of POD and TDC, the contents of vinpocetine and vincamine in the leaves of vincas, and the effects of arid + applied nitrogen (LN, HN) And vincristine accumulation. Conclusion: The effects of drought stress and nitrogen application on the activities of POD and TDC and the contents of four alkaloids in leaves of Vinca were significantly different. Under mild drought stress, the effect of applying nitrogen (LN) to vinorelbine, vinblastine, Vincristine, vincristine most significant increase in content, through the total nitrogen content and enzyme activity to regulate.