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目的:探讨沥青烟对作业工人淋巴细胞周期及肿瘤标志水平的影响。方法:选择某炭素厂沥青熔化工段职工31名作为观察组,另外选择该厂行政管理和后勤部门职工27名作为对照组,流式细胞术检测外周血淋巴细胞周期及DNA倍体,电化学发光法检测血清非小细胞癌抗原(CYFRA21-1)、神经元特异性烯醇化酶(NSE),放射免疫分析法检测癌胚抗原(CEA)、甲胎蛋白(AFP)水平。结果:观察组G0/G1、S期细胞所占比例分别为(93.37±1.53)%、(3.33±3.00)%,小于对照组(94.80±0.85)%、(3.40±1.41)%,观察组G2/M期细胞所占比例为(3.30±1.39)%,大于对照组(1.80±2.26)%,差异均无统计学意义,P>0.05。观察组细胞增殖指数(PI)、DNA相对含量(DNARC)值分别为0.07±0.02、209.93±0.50,高于对照组0.05±0.01、207.00±3.11,差异无统计学意义,P>0.05。血清CEA、NSE、AFP和CYFRA21-1水平分别为9.98±1.17、5.03±3.39、28.92±10.25和1.71±1.46,高于对照组8.55±1.51、3.24±2.00、23.85±14.93和0.84±0.50,差异无统计学意义,P>0.05。结论:观察外周血淋巴细胞各周期细胞数及血清肿瘤标志水平改变,尚不能确定沥青烟是否对作业工人外周血淋巴细胞周期及肿瘤标志水平有影响。
Objective: To investigate the impact of Asphalt-induced smoking on lymphocyte cycle and tumor marker in workers. Methods: Totally 31 employees in the asphalt melting section of a carbon plant were selected as the observation group. In addition, 27 workers in the administrative department and logistics department of the plant were selected as the control group. Flow cytometry was used to detect the peripheral blood lymphocyte cycle and DNA ploidy. Electrochemiluminescence Methods The serum levels of non-small cell carcinoma antigen (CYFRA21-1), neuron-specific enolase (NSE) and carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) were detected by radioimmunoassay. Results: The percentage of G0 / G1 and S phase cells in observation group was (93.37 ± 1.53)% and (3.33 ± 3.00)% respectively, which was lower than that in control group (94.80 ± 0.85)% and (3.40 ± 1.41)% / M phase cells was (3.30 ± 1.39)%, which was significantly higher than that of the control group (1.80 ± 2.26)%, the difference was not statistically significant (P> 0.05). The proliferation index (PI) and the relative content of DNA (DNARC) in the observation group were 0.07 ± 0.02 and 209.93 ± 0.50, respectively, which were higher than those of the control group (0.05 ± 0.01 and 207.00 ± 3.11), with no significant difference (P> 0.05). Serum levels of CEA, NSE, AFP and CYFRA21-1 were 9.98 ± 1.17, 5.03 ± 3.39, 28.92 ± 10.25 and 1.71 ± 1.46, respectively, higher than the control group 8.55 ± 1.51,3.24 ± 2.00,23.85 ± 14.93 and 0.84 ± 0.50, respectively No statistical significance, P> 0.05. CONCLUSION: It is not yet possible to determine if asparagus smoke affects the peripheral blood lymphocyte cell cycle and tumor marker level of workers in peripheral blood.