实时荧光定量聚合酶链反应技术是一种基于荧光共振能量转移原理进行核酸定量的新型技术,常用荧光检测系统主要包括SYBR Green I染料、Taqman探针、分子信标探针、蝎子引物探针、杂交探针、LUX~(TM)引物、Amplisensor探针等。目前该技术已成功应用于胚胎植入前遗传学诊断的性别鉴定、基因型分析、基因突变检测和基因表达研究等方面,具有潜在的应用价值。 Real-time fluorescence quantitative polymerase chain reaction is a new technique based on the principle of fluorescence resonance energy transfer for nucleic acid quantification. Common fluorescence detection systems include SYBR Green I dye, Taqman probe, molecular beacon probe, scorpion primer probe, Hybridization probe, LUX ™ primer, Amplisensor probe and the like. At present, the technology has been successfully applied in the preimplantation genetic diagnosis of gender identification, genotyping, gene mutation detection and gene expression studies, has potential applications.