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新生牛主动脉分离获得单个内皮细胞培养于微孔滤膜上直至形成致密单层。通过灌流含 5 g/L白蛋白的Hanks液后 ,测定经低渗溶液处理 (将M1 99溶液中含的 2 0 %血清改成 2 %血清 )或经低渗溶液加 1 0 4g/ml黄芪总黄酮或 1 0 4g/ml黄芪毛蕊异黄酮处理的液体滤过系数 (Kf)、液体滤过流量 (Jv)和蛋白质渗透压反射系数 (σ)。内皮单层经低渗溶液处理后 ,Kf和Jv降低 ,σ升高 ;而总黄酮和毛蕊异黄酮能抑制因低渗造成的Kf和Jv的降低及σ升高。形态学分析表明总黄酮、毛蕊异黄酮能够抑制因低渗造成细胞肿胀和细胞间距离的增宽。结果表明低渗能够引起血管内皮单层通透性的增加 ,而黄芪黄酮能够减轻低渗造成的内皮单层通透性的增加。
Neonatal bovine aorta was isolated and a single endothelial cell was cultured on the microporous filter until a dense monolayer was formed. After perfusion of Hanks’ solution containing 5 g/L albumin, the solution was treated with hypotonic solution (20 % serum contained in M1 99 solution was changed to 2 % serum) or hypotonic solution plus 10 4 g/ml jaundice. Total liquid flavonoids or 10 4 g/ml jaundice flavone treatment fluid filtration coefficient (Kf), liquid filtration flow rate (Jv), and protein osmotic pressure reflection coefficient (σ). Endothelial monolayers were treated with hypotonic solution. Kf and Jv decreased and σ increased. Total flavonoids and causalin inhibited the decrease of Kf and Jv and the increase of σ due to hypotonicity. Morphological analysis indicated that total flavonoids and causal isoflavones inhibited swelling and widening of the intercellular distance due to hypotonicity. The results showed that hypotonicity can increase the permeability of vascular endothelial monolayer, and Scutellariae flavonoids can reduce the increase of permeability of endothelial monolayer caused by hypotonicity.