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目的研究转化生长因子β1(TGF-β1)在糖尿病小猪肾组织中的表达及中药通心络对其表达的影响。方法以联合注射链尿佐菌素(STZ)与四氧嘧啶的方式复制糖尿病小猪模型,将动物随机分为糖尿病组(DM),通心络治疗组(TXL)和正常对照组(C)。6个月后处死动物,测量肾重/体重比值,应用免疫组化技术和western blotting技术观察肾组织中TGF-β1的表达。结果与对照组相比,糖尿病组小猪肾重/体重比值和TGF-β1的表达明显增加,通心络可显著降低肾重/体重比值和TGF-β1的表达。结论通心络通过影响糖尿病小猪肾组织中TGF-β1的表达改善糖尿病肾组织纤维化。
Objective To study the expression of transforming growth factor-β1 (TGF-β1) in the kidney of diabetic piglets and the effect of Tongxinluo on its expression. Methods The diabetic piglets were duplicated by injection of streptozotocin (STZ) and alloxan. The animals were randomly divided into diabetic group (DM), TXL group and normal control group (C) . After 6 months, the animals were sacrificed and the ratio of kidney weight to body weight was measured. The expression of TGF-β1 in renal tissues was observed by immunohistochemistry and western blotting. Results Compared with the control group, the kidney weight / body weight ratio and the expression of TGF-β1 in diabetic piglets were significantly increased. Tongxinluo significantly reduced the ratio of kidney weight to body weight and the expression of TGF-β1. Conclusion Tongxinluo can improve diabetic renal fibrosis by affecting the expression of TGF-β1 in the kidney of diabetic pigs.