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目的探讨胃组织中基质金属蛋白酶-2(MMP-2)和T淋巴瘤侵袭转移诱导因子1(Tiam1)基因的表达与胃癌生物学行为的相关性。方法应用S-P免疫组化法,对40例胃癌手术切除标本进行抗人MMP-2单克隆抗体和Tiam1多克隆抗体免疫组化染色,检测癌组织、癌旁组织、手术切缘区正常组织各区域MMP-2和Tiam1蛋白表达,并分析二者之间及二者的表达与胃癌临床病理特征之间的关系。结果 MMP-2和Tiam1蛋白表达在癌组织与手术切缘区正常组织、癌组织与癌旁组织、癌旁组织与正常手术切缘区组织之间差异均具有显著性。癌组织中MMP-2和Tiam1蛋白表达在患者性别、年龄之间无显著性差异,而与肿瘤分化程度、浸润深度、淋巴结及远处转移、临床TNM分期均呈正相关;且两种蛋白表达彼此间具有相关性,即同时表达阳性的胃癌病例具有更为恶性的生物学特征。结论MMP-2和Tiam1在胃癌的发生和侵袭转移中可能起着重要作用,检测MMP-2和Tiam1的表达有望成为判断胃癌病变发展、预测肿瘤转移潜能的客观指标。
Objective To investigate the relationship between the expression of matrix metalloproteinase-2 (MMP-2) and Tiam1 in gastric cancer and the biological behavior of gastric cancer. Methods SP immunohistochemical method was used to detect the expression of anti-human MMP-2 monoclonal antibody and Tiam1 polyclonal antibody in 40 cases of gastric cancer. Immunohistochemistry was used to detect the expression of tumor tissue, peritumoral tissue, MMP-2 and Tiam1 protein expression, and analyze the relationship between the two and their expression with the clinicopathological features of gastric cancer. Results The expressions of MMP-2 and Tiam1 were significantly different between the normal tissues, the normal tissues in the margin of the surgical margins, the adjacent tissues of the cancerous tissues and the adjacent tissues of the paracancerous tissues and the normal margins of the surgical margins. The expression of MMP-2 and Tiam1 protein in cancer tissues had no significant difference between the sex and age of the patients, but positively correlated with the degree of tumor differentiation, depth of invasion, lymph node and distant metastasis, clinical stage of TNM, and the expression of both proteins Between the correlation, that is, positive cases of gastric cancer have more malignant biological characteristics. Conclusions MMP-2 and Tiam1 may play an important role in the occurrence and invasion of gastric cancer. Detecting the expression of MMP-2 and Tiam1 is expected to be an objective index for predicting the development of gastric cancer and predicting the metastatic potential.