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我们在第一篇文章中报导了老龄家兔免疫指标测定结果。但是,做为衰老指标,免疫指标只是一部分。本文报导老龄家兔体内自由基等指标观察结果。材料与方法一、试验动物三个月大耳白家兔(青年组),四年右右大耳白家兔(老龄组)。二、自由基测定分别取家兔大脑、胸腺、脾脏、肝脏适量,置玻璃匀浆器中充分磨碎,过滤后加生理盐水至9ml,即为待测浆液。取待测浆液3ml,加入鲁米诺增敏剂3ml,用RM—2型晶溶发光测定仪测定发光强度(脉冲数/分)。三、脂褐素测定按Sohal R.S.的方法,取家兔脑组织适量,置玻璃匀浆器中,加入2ml蒸馏水,磨碎,加入氯仿—甲醇液7ml,充分振荡,离心,取氯仿层。用荧光光度计(HI-
In the first article, we reported the results of the immunoassay in elderly rabbits. However, as an indicator of aging, the immune index is only part of it. This article reports the elderly rabbits free radicals and other indicators observed. Materials and Methods First, the experimental animals three months old white rabbits (youth group), four years of large white rabbits (aging group). Second, determination of free radicals were taken rabbit brain, thymus, spleen, liver amount, set the glass homogenizer fully grated, filtered and then add saline to 9ml, is the test serum. Take the test solution 3ml, adding luminol sensitizer 3ml, with the RM-2 type crystal luminescence analyzer determination of luminous intensity (pulse number / min). Determination of lipofuscin By SohalR.S. method, take the amount of brain tissue in rabbits, set the glass homogenizer, add 2ml of distilled water, ground, add chloroform - methanol solution 7ml, shaking well, centrifuged, and the chloroform layer. Using a fluorometer (HI-