盘龙参内生真菌S-3可产生黄酮类化合物,通过ITS法测序分析,S-3鉴定为嗜松青霉Penicillium pinophilum。为了增加内生真菌S-3产黄酮量,以单因素结合正交法优化S-3菌株发酵条件,结果表明,最佳发酵条件如下:发酵培养基组成为马铃薯100 g,玉米粉40 g,葡萄糖10 g,豆饼粉10 g,磷酸二氢钾3 g,硫酸镁1.5 g,水1 000 mL,pH6.7;发酵温度28℃,摇床转数160 r/min,发酵时间4 d。 Panax ginseng endophytic fungi S-3 can produce flavonoids, by ITS sequencing analysis, S-3 identified Penicillium pinophilum Penicillium. In order to increase the flavonoid content of endophytic fungi S-3, the fermentation conditions of S-3 strain were optimized by single factor orthogonal test. The results showed that the optimum fermentation conditions were as follows: fermentation medium consisted of 100 g potato, 40 g corn flour, Glucose 10 g, soybean meal 10 g, potassium dihydrogen phosphate 3 g, magnesium sulfate 1.5 g, water 1000 mL, pH 6.7; fermentation temperature 28 ℃, shaking speed 160 r / min, fermentation time 4 d.