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以咪唑-1-乙酸改性壳聚糖(IACS)为生物支架,在模拟体液(SBF)中调控仿生合成羟基磷灰石(HA)。探讨了支架类型、陈化时间、钙离子(Ca~(2+))浓度等因素对IACS生物支架在SBF中调控合成HA的影响,并通过X射线衍射仪、傅里叶变换红外光谱分析仪、场发射扫描电子显微镜等对合成的HA进行表征。结果表明,在SBF中,以500KDIACS1为生物模板支架,Ca~(2+)离子浓度为0.10mol/L,反应温度为37℃,随着陈化时间从12h延长至72h,HA从球型颗粒向针状转化且分散越均匀,与自然骨中的HA相类似。因此,利用IACS生物支架调控合成的HA有望在硬组织修补与替换、药物缓释等生物医学工程领域得到广泛应用,也为仿生合成其他生物矿物提供借鉴。
Using imidazole - 1 - acetic acid modified chitosan (IACS) as biological scaffold, biomimetic hydroxyapatite (HA) is regulated in simulated body fluid (SBF). The effects of scaffold type, aging time and Ca (2+) concentration on the regulation of synthetic HA in SBF were investigated by X-ray diffractometry, Fourier transform infrared spectroscopy , Field emission scanning electron microscopy, etc. The synthesized HA was characterized. The results showed that in the SBF, 500KDIACS1 was used as a bio-template scaffold, the concentration of Ca 2+ was 0.10mol / L, the reaction temperature was 37 ℃, and the HA was evolved from 12h to 72h with aging time. Converted to acicular shape and dispersed more evenly, similar to HA in natural bones. Therefore, HA synthesized by IACS biological scaffolds is expected to be widely used in the field of biomedical engineering such as hard tissue repair and replacement, drug sustained-release and other biological minerals.