论文部分内容阅读
原位分子杂交法是近年来发展较快的又一新技术,已被广泛地应用于检测细胞或组织切片中病毒基因和肿瘤基因.1984年Blum等首次报道了把原位杂交技术与免疫组织化学方法相结合应用~3H标记探针在肝石蜡切片上同时检测乙型肝炎病毒DNA(HBV-DNA)和乙型肝炎核心抗原(NBcAg).从而使在同一组织切片上观察病毒及其表达产物的定位和分布关系成为可能.目前尚未见到其它类似的报道.我们按Blum的
In situ hybridization is another rapid development in recent years, a new technology has been widely used in the detection of cell or tissue sections of the viral genes and tumor genes. Blum et al 1984 reported for the first time in situ hybridization and immunohistochemistry Chemical method combined with ~ 3H labeled probe in the liver paraffin sections simultaneously detect hepatitis B virus DNA (HBV-DNA) and hepatitis B core antigen (NBcAg) .Thus make the same tissue sections observed virus and its expression product The positioning and distribution of possible. There have been no other similar reports. We press the Blum