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目的:探讨非编码RNA MIR155HG对人肺癌A549细胞增殖、迁移和侵袭能力的影响及机制。方法:过表达或敲减MIR155HG后,MTS检测A549细胞生长的变化,流氏细胞仪检测细胞周期。Transwell迁移和侵袭实验检测过表达或敲减MIR155HG后,A549细胞迁移和侵袭能力的变化。过表达MIR155HG后,定量PCR检测A549细胞中mi R-155-5p的表达。结果:MTS法显示,转染72 h和96 h后,过表达MIR155HG组细胞吸光度(absorbance,A)值分别为(2.47±0.14)和(3.13±0.15),均较对照组[(2.09±0.12)(72 h)和(2.50±0.13)(96 h)]增加(P=0.006,P=0.027);敲减MIR155HG组细胞在48、72和96 h的OD值分别为(1.69±0.15),(1.87±0.09)和(2.24±0.16),较对照组[(2.04±0.06)(48 h),(2.43±016)(72 h)和(2.88±0.11)(96 h)]均降低(P=0.006,P=0.006和P=0.004);敲减MIR155HG组的A549细胞G0/G1期比例为63.93%,与对照组(54.32%)相比,增加了9.61%;迁移实验结果显示,过表达MIR155HG组的穿膜细胞数为(31.67±3.51)个,与对照组(12.67±2.51)相比增加明显(P=0.002)。敲减MIR155HG组的A549细胞的穿膜细胞数为(13.67±3.21)个,与对照组(36.00±4.58)相比明显减少(P=0.002)。侵袭实验结果显示,过表达MIR155HG组细胞穿膜细胞数为(42.33±7.02)个,与对照组(15.67±3.51)相比明显增加(P=0.004)。敲减MIR155HG组穿膜细胞数为(15.00±3.60)个,与对照组(39.00±4.36)相比明显减少(P=0.002)。定量PCR显示,过表达MIR155HG后,mi R-155-5p的表达较对照组增加的倍数为(17.99±2.42)(P=0.000)。结论:MIR155HG能增强A549细胞的增殖、迁移和侵袭能力,其发挥作用的机制可能是通过产生mi R-155-5p来实现。
Objective: To investigate the effect and mechanism of non-coding RNA MIR155HG on the proliferation, migration and invasion of human lung cancer A549 cells. Methods: After MIR155HG overexpression or knockdown, the growth of A549 cells was detected by MTS. Flow cytometry was used to detect the cell cycle. Transwell migration and invasion assays were used to detect the migration and invasion ability of A549 cells after overexpression or knockdown of MIR155HG. After overexpression of MIR155HG, the expression of mi R-155-5p in A549 cells was detected by quantitative PCR. Results: MTS assay showed that the absorbance (A) values of MIR155HG overexpression group were (2.47 ± 0.14) and (3.13 ± 0.15), respectively, compared with those in control group [(2.09 ± 0.12 (P = 0.006, P = 0.027). The OD value of MIR155HG knockdown cells at 48, 72 and 96 h were (1.69 ± 0.15), (1.87 ± 0.09) and (2.24 ± 0.16) in the control group were significantly lower than those in the control group [(2.04 ± 0.06), (48 h), (2.43 ± 016, 72 h and (2.88 ± 0.11, 96 h] = 0.006, P = 0.006, P = 0.004). The percentage of G0 / G1 phase in A549 cells knocked down by MIR155HG was 63.93%, which was 9.61% higher than that in control group (54.32%). The results of migration assay showed that overexpression The number of transmembrane cells in MIR155HG group was (31.67 ± 3.51), which was significantly higher than that in control group (12.67 ± 2.51) (P = 0.002). The number of transmembrane cells in A549 cells knocked down by MIR155HG group was (13.67 ± 3.21), which was significantly lower than that in control group (36.00 ± 4.58) (P = 0.002). The results of invasion assay showed that the number of transmembrane cells in MIR155HG overexpression group was (42.33 ± 7.02), which was significantly higher than that in control group (15.67 ± 3.51) (P = 0.004). The number of transmembrane cells knocked down in MIR155HG group was (15.00 ± 3.60), which was significantly lower than that in control group (39.00 ± 4.36) (P = 0.002). Quantitative PCR showed that the expression of mi R-155-5p was (17.99 ± 2.42) times higher than that of the control group (P = 0.000) after overexpression of MIR155HG. Conclusion: MIR155HG can enhance the proliferation, migration and invasion of A549 cells. The mechanism may be through the production of mi R-155-5p.