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目的:探讨中药复方糖耐康对2型糖尿病KKAy小鼠血脂代谢及其早期肾脏病理改变的影响。方法:12周龄雄性KKAy小鼠50只按血糖随机分为模型组、缬沙坦组、糖耐康高、中、低剂量组,另设10只C57BL/6J小鼠为正常组,模型组和正常组给予0.9%氯化钠溶液,其余组给予对应浓度的药液灌胃,每2周检测体质量、空腹血糖,每4周测24h尿蛋白定量,8周后处死,检测血生化指标,观察肾脏病理变化,检测肾组织FN mRNA表达。结果:与同龄正常组小鼠比,模型组小鼠体质量显著增加(P<0.01),各给药组较模型组同期体质量有显著下降(P<0.05,P<0.01):模型组较正常组小鼠的空腹血糖(FBG)、血甘油三脂(TG)、血总胆固醇(TC)、血胰岛素(TNS)显著升高(P<0.01),糖脂代谢紊乱;24h尿蛋白定量、肾重升高(P<0.05),20周龄模型组小鼠肾小球较正常组增大,细胞外膜基质增加,肾小管管腔扩大,蛋白管型明显,半定量分析肾组织病理损伤评分增大,RT-PCR定量分析肾组织FN mRNA表达显著增加(P<0.05);各用药组经缬沙坦、糖耐康治疗后,上述指标均较模型组有明显改善(P<0.05)。结论:糖耐康可调节KKAy小鼠糖脂代谢紊乱,对早期肾脏病理改变有较好的保护作用,抑制FN mRNA沉积改善肾纤维化是其可能的作用机制之一。
Objective: To investigate the effect of Tangjikang, a traditional Chinese medicine compound, on blood lipid metabolism and early renal pathological changes in type 2 diabetic KKAy mice. Methods: Fifty male KKAy mice of 12 weeks old were randomly divided into model group, valsartan group, high, medium and low dose of glucose, and another 10 C57BL / 6J mice were selected as model group 0.9% sodium chloride solution was given to the rats in the normal group and the other rats were given the corresponding drug solution by gavage. The body weight and fasting blood glucose were measured every 2 weeks. The urine protein was measured every 4 weeks. After 8 weeks, The pathological changes of kidney were observed, and the expression of FN mRNA in renal tissue was detected. Results: Compared with normal mice of the same age, the body weight of mice in the model group increased significantly (P <0.01), the body weight of the mice in the model group decreased significantly (P <0.05, P <0.01) The fasting blood glucose (FBG), triglyceride (TG), total cholesterol (TC) and blood insulin (TNS) of normal mice were significantly increased (P <0.01) (P <0.05). Compared with the normal group, the glomeruli in the 20-week-old model group increased, the matrix of the extracellular matrix increased, the tubular lumen enlarged and the protein tube type was obvious. The pathological changes of renal tissue were semi-quantitatively analyzed (P <0.05). The levels of FN mRNA in renal tissue were significantly increased by RT-PCR (P <0.05). Compared with the model group, the above indexes were significantly improved after valsartan and glucose- . Conclusion: Tiandikang can regulate the disorder of glucose and lipid metabolism in KKAy mice and has a good protective effect on pathological changes of early kidney. Inhibition of FN mRNA deposition to improve renal fibrosis is one of its possible mechanisms.