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目的:用荷瘤兔模型研究乏氧显像剂(4,9diaza3,3,10,10tetramethyldodecan2,11dionedioxime,99mTcHL91)和18F氟代脱氧葡萄糖(18Ffluorodeoxyglucose,18FFDG)在肿瘤乏氧诊断中的应用价值。方法:1)体外试验,分别观察在常氧及乏氧状态下对VX2细胞摄取99mTcHL91和18FFDG的影响。2)体内试验,分别于耳缘静脉注入99mTcHL91后3h和18FFDG后1h,采集荷瘤兔的图象,随后,处死荷瘤兔取脑、肺、心、肝、脾、肾、坏死和非坏死肿瘤等标本称质量,测定放射性计数。肿瘤组织切片行HE染色,观察其特点。结果:1)乏氧与常氧状态下比较能引起VX2细胞对99mTcHL91和18FFDG的摄取并随时间延长而增加,P<0.05。2)荷瘤兔肿瘤显像清晰,肿瘤未坏死部位的放射性分别是坏死部位的4倍(99mTcHL91)和3.5倍(18FFDG)。HE染色显示瘤内有大量的坏死组织。结论:乏氧能引起VX2细胞对99mTcHL91和18FFDG的摄取增加;VX2荷瘤兔的瘤体在99mTcHL91和18FFDG显像上均为清晰的阳性病灶,坏死肿瘤组织的放射性低于非坏死肿瘤组织;两者均不能将乏氧的部位明确地显示出来。
OBJECTIVE: To investigate the value of hypoxia imaging agents (4,9diaza3,3,10,10tetramethyldodecan2,11dionedioxime, 99mTcHL91) and 18F fluorodeoxyglucose (18FFDG) in the diagnosis of tumor hypoxia. Methods: 1) In vitro experiments were conducted to observe the effects of 99mTcHL91 and 18FFDG on VX2 cells under normoxia and hypoxia respectively. 2) In vivo, the images of tumor-bearing rabbits were collected at 3h after injection of 99mTcHL91 and 1h after 18FFDG, respectively. Then the tumor-bearing rabbits were sacrificed for brain, lung, heart, liver, spleen, kidney, necrosis and non-necrosis Tumor and other specimens called quality, determination of radioactive counts. Tumor tissue sections were stained with HE to observe its characteristics. Results: 1) Under hypoxia and normoxia, the uptake of 99mTcHL91 and 18FFDG by VX2 cells increased with time, P <0.05. 2) The tumors of tumor-bearing rabbits were clearly visualized and the radioactivity of tumor-free necrotic sites It is 4 times (99mTcHL91) and 3.5 times (18FFDG) of necrotic sites. HE staining showed a large number of tumor necrosis. CONCLUSION: Hypoxia can induce the uptake of 99mTcHL91 and 18FFDG in VX2 cells. VX2 tumor-bearing rabbits showed clear positive lesions on 99mTcHL91 and 18FFDG images, while the necrotic tumor tissues showed lower radioactivity than non-necrotic tumor tissues. Hypoxia can not be clearly demonstrated by the site.