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以苗期抗旱性不同的37份六倍体普通小麦(AABBDD)、3份A基因组材料(AA)和3份四倍体小麦(AABB)为材料,通过直接测序法检测TaCRT-A基因的单核苷酸多态性,分析该基因多态性与小麦苗期抗旱性的关系,并进行遗传定位.结果表明:TaCRT-A基因DNA长度为3887bp,在总长度为167141bp的核苷酸序列中共检测到202个核苷酸变异位点,其中包括165个SNP和37个InDel,二者出现的频率分别为1/1013bp和1/4517bp.编码区的核苷酸多样性π值小于非编码区,编码区所承受的选择压力较大.43份试材可分为14种单倍型,其中H1、H2和H13分别含有普通小麦二倍体野生近缘种A基因组供体种的1份材料,H6、H7分别包含抗旱性极强的1份材料,H8包含四倍体波斯小麦并同时包含抗旱材料与干旱敏感材料,H11主要包括强抗旱材料与中等抗旱材料;虽然TaCRT受水分胁迫诱导表达,但TaCRT-A基因的结构多态性分析未能揭示其多态性与小麦苗期抗旱性之间的直接关系;利用RIL群体(Opata 85×W7984)将该基因定位于3A染色体标记Xmwg30～Xmwg570之间,遗传距离分别为10.5cM和49.6cM.
A total of 37 hexaploid wheat (AABBDD), 3 A genome materials (AA) and 3 tetraploid wheat (AABB) with different drought resistance at seedling stage were used as materials to detect the single TaCRT-A gene by direct sequencing The results showed that the length of TaCRT-A gene was 3887bp and the total length was 167141bp in the nucleotide sequence A total of 202 nucleotide variation sites were detected, including 165 SNPs and 37 InDel, and their frequencies were 1 / 1013bp and 1 / 4517bp, respectively.Nucleotide diversity in the coding region was smaller than that in the non-coding region , And the selection pressure on the coding region is relatively large.43 samples can be divided into 14 haplotypes, among which H1, H2 and H13 contain 1 copy of A genome donor of common wheat diploid wild relatives , H6 and H7 respectively contain 1 material with strong drought resistance, H8 contains tetraploid Persian wheat and contains both drought-resistant material and drought sensitive material, and H11 mainly includes strong drought-resistant material and medium drought-resistant material. Although TaCRT is induced by water stress However, structural polymorphism analysis of TaCRT-A gene failed to reveal its polymorphism A direct relationship between the drought resistance of wheat seedlings; Using RIL population (Opata 85 × W7984) The gene is located on chromosome 3A Xmwg30 ~ Xmwg570 between markers, genetic distance of 10.5cM and 49.6cM.