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目的 了解高眼压状态下视网膜 N-甲基 - D-天门冬氨酸受体 (N- methyl- D- aspartatereceptor,NMDAR)的功能亚单位 NMDAR1基因 m RNA表达的情况。 方法 采用逆转录多聚酶链反应(reverse transcription polymerase chain reaction,RT- PCR)、β-肌动蛋白 (β- actin)作内对照对高眼压模型眼视网膜内 NMDAR1基因的 m RNA进行半定量分析。16只大白兔的 2 6只眼分成 3组。实验组 10只兔 10只眼 ,前房穿刺生理盐水高压 6 0 mm Hg(1mm Hg=0 .133k Pa)灌注 ,持续 4h。阳性对照组 10只兔 10只眼 ,为实验组的对侧眼 ,用实验组相同的方法以 2 0 mm Hg的压力前房灌注持续 4h作阳性对照。正常对照组 6只兔 6只眼 ,不作眼球灌注处理。 结果 实验组的高眼压灌注眼与阳性对照眼 NMDAR1的 m RNA的水平无明显差异 (P>0 .0 5 )。 结论 急性高眼压未改变兔视网膜 NMDAR1基因的 m RNA表达水平。
Objective To investigate the expression of NMDAR1 m RNA in functional subunit of retinal N-methyl-D-aspartate receptor (NMDAR) under ocular hypertension. Methods Reverse transcription polymerase chain reaction (RT-PCR) and β-actin (β-actin) were used as internal controls to semi-quantitatively analyze the m RNA of NMDAR1 gene in the retina of ocular hypertension model. Twenty-six rabbits in 16 rabbits were divided into 3 groups. In the experimental group, 10 rabbits were injected with 60 mm Hg saline (1 mm Hg = 0.133 kPa) for 4 h in 10 eyes of anterior chamber. Positive control group of 10 rabbits 10 eyes, the contralateral eye for the experimental group, with the same experimental group with 20 mm Hg pressure anterior chamber perfusion for 4h as a positive control. The normal control group, 6 rabbits, 6 eyes, not for eyeball perfusion. Results There was no significant difference in the level of m RNA between NMDAR1 and experimental group (P> 0.05). Conclusions Acute intraocular pressure does not change the mRNA expression of NMDAR1 in rabbit retina.