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1991年Christou等用基因枪法获得了转基因水稻植株[1];1994年Hiei等建立农杆菌介导的高效水稻转化体系[2],此后育种家们又相继获得了各类转基因水稻植株,并进一步验证了外源基因的遗传表达效果。但转基因技术本身以及转基因材料在生产上的应用尚存在许多问题。①基因枪法、
In 1991, Christou et al. Obtained transgenic rice plants by particle gun method [1]. In 1994, Hiei et al. Established an Agrobacterium-mediated efficient rice transformation system [2], after which breeders have successively obtained all kinds of transgenic rice plants and further The genetic expression of exogenous genes was verified. However, there are still many problems in the application of genetically modified technology and the production of transgenic materials. ① gene gun method,