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目的:观察大豆异黄酮对60Co γ射线引起小鼠肝组织过氧化损伤的影响。方法:实验根据体质量将80 只雌性昆明小鼠随机均分为5组,即正常对照、阳性对照(单纯辐照组)及低、中、高剂量大豆异黄酮实验组(50、100、400 mg/kg)。辐照前正常对照、阳性对照组及实验组每天分别以溶剂0.5%羧甲基纤维素钠(CMC Na)和不同剂量大豆异黄酮连续灌胃14 d,灌胃至第7天,除正常对照组外,其余各组小鼠均接受4.56 Gy 60Co γ全身性照射1次,照射后继续灌胃2 d及7 d后杀鼠取肝组织作生化分析。结果:照射后第2天100、400 mg/kg大豆异黄酮组及照射后第7 天3 个浓度大豆异黄酮组显著提高肝细胞质过氧化氢酶的活性(P<0.05);照射后第7天100 mg/kg大豆异黄酮组的肝组织谷胱甘肽过氧化物酶活性有显著提高(P<0.05);照射后第2天50 mg/kg大豆异黄酮使肝组织总超氧化物歧化酶活性显著下降(P<0.05),其余各组间无显著差异;照射后第7天100 mg/kg大豆异黄酮使肝组织丙二醛含量下降,与对照组有显著差异(P<0.05),照射后第2 天各照射组肝组织丙二醛含量有一过性升高,但第7天实验组已降至正常水平。结论:大豆异黄酮可提高受照小鼠肝组织的抗氧化能力,但不呈量效关系。
Objective: To observe the effect of soy isoflavones on the liver injury caused by 60Co γ ray in mice. Methods: Eighty female Kunming mice were randomly divided into five groups: normal control group, positive control group (simple irradiation group) and low, medium and high dosage soy isoflavones group (50, 100, 400 mg / kg). Normal control before irradiation, the positive control group and the experimental group were treated with 0.5% sodium carboxymethyl cellulose (CMC Na) and different doses of soy isoflavones daily for 14 days, Group, the rest of the mice in each group received 4.5 Gy Gy 60Co γ systemic irradiation once. After irradiation, the mice were sacrificed on the 2nd and 7th days after irradiation for biochemical analysis. Results: In the soy isoflavones group at 100, 400 mg / kg on the second day after irradiation and at the 7th day after irradiation, the soy isoflavones significantly increased the activities of hepatic cytoplasmic catalase (P <0.05) The activity of glutathione peroxidase in the liver tissue of 100 mg / kg soy isoflavone group was significantly increased (P <0.05) on day 2; 50 mg / kg soy isoflavone on the second day after irradiation caused the total superoxide dismutase (P <0.05), and there was no significant difference between the other groups. On the 7th day after irradiation, 100 mg / kg soy isoflavones decreased the content of malondialdehyde in liver tissue, which was significantly different from the control group (P <0.05) On the second day after irradiation, the malondialdehyde content of liver tissue in each irradiation group increased transiently, but on the seventh day, the experimental group had dropped to the normal level. Conclusion: Soy isoflavones can improve the anti-oxidative ability of liver in irradiated mice, but not in a dose-response relationship.