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在模拟人体生理条件下,利用荧光、紫外?可见吸收和同步荧光光谱法研究了水溶性壳聚糖(CS)及两种壳聚糖稀土配合物(CS-La和CS-Dy)与牛血清白蛋白(BSA)的相互作用.紫外吸收光谱分析表明,CS,CS-La和CS-Dy相互作用的最大吸收峰强度都增加.用Stern–Volmer方程分别对荧光光谱的实验数据进行分析,发现CS-La对BSA的荧光猝灭作用属于静态荧光猝灭,反应生成新的复合物.而CS和CS-Dy对BSA的荧光猝灭作用有静态荧光猝灭和动态猝灭,发生了分子内的非辐射能量转移.求得三种物质在室温和体温的情况下与BSA相互作用过程的结合常数KA[291 K,CS-BSA:1.807?104 L/(g·s),CS-La-BSA:3.065?104 L/(g·s),CS-Dy-BSA:2.193?104 L/(g·s);310 K,CS-BSA:2.665?104 L/(g·s),CS-La-BSA:2.022?104 L/(g·s),CS-Dy-BSA:7.246?104 L/(g·s)]和相关热力学参数;确定了CS和CS-Dy与BSA之间的主要作用力是静电和疏水作用力,而CS-La与BSA之间的作用力主要为范德华力和氢键等作用力.同步荧光光谱表明,CS,CS-La和CS-Dy均对牛血清白蛋白的构象和所处的微环境产生影响.
Under simulated human physiological conditions, the effects of water-soluble chitosan (CS) and two chitosan rare earth complexes (CS-La and CS-Dy) with bovine serum (BSA) .Ultraviolet absorption spectroscopy analysis showed that the maximum absorption peak intensities of CS, CS-La and CS-Dy interaction increased.According to the Stern-Volmer equation, the experimental data of fluorescence spectra were analyzed respectively and found that The fluorescence quenching of BSA by CS-La belongs to the static fluorescence quenching reaction, resulting in the formation of a new complex, while the fluorescence quenching of CSA and CS-Dy by BSA quenching static fluorescence quenching and dynamic quenching occurred intramolecular The binding constants KA [291 K, CS-BSA: 1.807-104 L / (g · s), CS-La- BSA: 3.065-104 L / (g · s), CS-Dy-BSA: 2.193-104 L / (g · s); 310 K, CS- La-BSA: 2.022 104 104 L / (g · s), CS-Dy-BSA: 7.246 104 104 L / (g · s)] and related thermodynamic parameters. The force is electrostatic and hydrophobic forces, while the main force between CS-La and BSA is Der Waals forces and hydrogen bond forces. By synchronous fluorescence spectroscopy, CS, CS-La and CS-Dy have an impact on the microenvironment in which the conformation and bovine serum albumin.