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目的采用果蝇伴性隐性致死实验(Sex-Linked Recessive Lethal Test,SLRL Test),对酞酸二丁酯(di-n-butyl phthalate,DBP)&酞酸二(2-乙基己基)酯(di〔2-ethylhexyl〕phathalate,DEHP)进行遗传毒性研究。方法用以1∶5混合的3个不同浓度DBP&DEHP染毒物对果蝇进行喂饲,染毒浓度分别为0.125、1和8 g/L。观察DBP&DEHP对SLRL实验不同阶段生殖细胞的致突变作用结果和雄蝇各生殖阶段不育率的影响。结果0.125 g/L浓度组第Ⅱ窝、1 g/L浓度组第Ⅱ、Ⅲ窝及8 g/L浓度组各阶段的突变率与对照组比较差异有统计学意义。第Ⅱ窝的致死突变率与受试浓度之间存在剂量-反应关系;而较高的2个浓度组亲代及F1代的不育率均较对照组有显著性提高(P<0.05,P<0.01)。结论在本试验条件下,结果提示DBP&DEHP对果蝇生殖细胞具有潜在的遗传毒性作用。
OBJECTIVE To investigate the effects of di-n-butyl phthalate (DBP) and bis (2-ethylhexyl) phthalate using Sex-Linked Recessive Lethal Test (SLRL Test) (di [2-ethylhexyl] phathalate, DEHP) for genotoxicity studies. METHODS Drosophila was fed with 3 different concentrations of DBP & DEHP in a 1: 5 mixture at concentrations of 0.125, 1 and 8 g / L, respectively. To observe the effect of DBP & DEHP on germ cell germ cell mutagenesis in different stages of SLRL experiment and the effects of DBP and DEHP on the male sterility of each stage of reproductive stage. Results The mutation rate in each group of Ⅱ, Ⅲ litter, and 8 g / L of group Ⅱ, 1 g / L at concentration of 0.125 g / L was significantly different from that of control group. There was a dose-response relationship between the lethal mutation rate and the test concentration in the second litter, while the male sterility rate in the two higher concentration groups was significantly higher than that in the control group (P <0.05, P < 0.01). Conclusions Under the experimental conditions, the results suggest that DBP & DEHP has a potential genotoxic effect on Drosophila germ cells.