目前,高速液相层析的检测器有用示差折光法、紫外及可见光吸光光度法、萤光光度法、库仑检测法、火焰电离检测法和电导度法等。其中示差折光法可用于任何化合物,但灵敏度较低,易受温度影响,尤其梯度洗脱时应用有困难。因此,高灵敏度的机动态性能好的紫外检测器最常用。紫外检测器一般多 Currently, high-speed liquid chromatography detector useful differential refractometry, UV and visible absorption spectrophotometry, fluorescence spectrophotometry, coulometry, flame ionization detection and conductivity method. Refractive index method which can be used for any compound, but lower sensitivity, susceptible to temperature effects, especially when applied gradient elution difficulties. Therefore, the high sensitivity of the dynamic performance of the UV detector is most commonly used. UV detector generally more