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目的通过对天津地区自然人群、动物宿主和媒介生物进行研究,以确定该地区是否为莱姆病疫源地。方法采用分层整群抽样方法选取3个代表性地区10个调查点的居民进行人群的莱姆病血清流行病学调查,同时用间接免疫荧光试验、病原分离培养和PCR方法对动物宿主和媒介生物进行血清学和病原学研究。并通过测序确定基因型。结果905份人群血清样本检测结果显示该地区人群的莱姆病感染率为5.97%;100份野鼠血清样本的感染率是32.00%,并从大林姬鼠、社鼠、小林姬鼠的脾脏和/或肾脏中检查到莱姆病螺旋体的特异片段。经聚类分析定为Borrelia garinii基因型,因此可以推断鼠类可能为此地区莱姆病螺旋体的重要储存宿主。共收集到1226只蜱,以长角血蜱为优势种。选取300只长角血蜱经PCR检测,其带菌率为4.67%(14/300)。结论首次发现天津蓟县山林地区可能为我国莱姆病的疫源地之一。
Objective To study whether the area is the source of Lyme disease by studying natural populations, animal hosts and vector organisms in Tianjin area. Methods The stratified cluster sampling method was used to select the residents of 10 survey sites in 3 representative areas for serological epidemiological investigation of Lyme disease in the population. Meanwhile, indirect immunofluorescence assay, pathogen isolation and culture, Biology for serological and etiological studies. Genotypes were determined by sequencing. Results The serum samples from 905 human populations showed that the prevalence of Lyme disease in this population was 5.97%. The infection rate of 100 samples of wild rat serum was 32.00%. The infection rate of Lyme disease among the populations of Apodemus, Rhesus, Apodemus and / Or kidneys checked Lyme disease spirochete specific fragments. The clustering analysis identified the Borrelia garinii genotype, thus suggesting that the murine may be an important storage host for Borrelia burgdorferi in this region. A total of 1226 ticks were collected, and H. longicornis was the dominant species. A total of 300 H. longicornis ticks were detected by PCR, and the carrier rate was 4.67% (14/300). Conclusion It is the first time that the forest area in Jixian, Tianjin may be one of the foci of Lyme disease in our country.