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目的研究香附对川芎中阿魏酸(ferulic acid,FA)在正常及偏头痛模型大鼠体内的药代动力学影响。方法用硝酸甘油(10mg/kg)制作偏头痛大鼠模型,SD大鼠分为正常川芎组、正常川芎-香附组、模型川芎组、模型川芎-香附组,灌胃给药,采用高效液相色谱法(HPLC)检测血药浓度,流动相为0.2%甲酸水(A)-甲醇(B),梯度洗脱,检测波长为322nm,检测给药后0~8h内大鼠血浆中FA的浓度,DAS 2.0软件计算药代动力学参数。结果 HPLC法的方法学考察均符合测定要求。正常组中川芎配伍香附后FA的吸收半衰期t1/2Ka缩短(P<0.05),达峰时间Tmax提前(P<0.05),但其他药代动力学参数与配伍前比较差异无统计学意义。模型组中川芎配伍香附后FA的达峰浓度Cmax、药时曲线下面积AUC(0-t)、分布半衰期t1/2α、表观分布容积增大(P<0.01或P<0.05),Tmax提前(P<0.05),清除率降低(P<0.01)。结论在正常大鼠体内香附加快了FA的吸收,而在偏头痛模型大鼠体内香附减慢FA在体内的分布和代谢,延长药物在体内的作用时间,从而提高了FA的生物利用度。
Objective To investigate the pharmacokinetics of ferulic acid (FA) in Ligusticum chuanxiong Hort in normal and migraine rats. Methods The rat model of migraine was made with nitroglycerin (10mg / kg). The SD rats were divided into normal Chuanxiong group, Chuanxiong - Cyperus rotundus group, Chuanxiong group and Chuanxiong - The plasma concentration was determined by liquid chromatography (HPLC). The mobile phase consisted of 0.2% formic acid in water (A) and methanol (B). The detection wavelength was 322 nm. The DAS 2.0 software calculates pharmacokinetic parameters. Results The methodological study of HPLC method met the determination requirements. The absorption half-life (t1 / 2Ka) of FA in the normal group was shorter than that of the control group (P <0.05), and the peak time of Tmax was earlier (P <0.05). However, there was no significant difference in other pharmacokinetic parameters between the two groups. In the model group, the maximal peak concentration Cmax of FA, the peak area AUC (0-t), the half-life t1 / 2α, the apparent volume of distribution (P <0.01 or P <0.05) Early (P <0.05), clearance rate decreased (P <0.01). CONCLUSIONS: Fragrance in normal rats accelerates the absorption of FA, while in migraine model rats, Cyperus rotundus decreases the distribution and metabolism of FA in the body and prolongs the action time of drugs in the body, thus improving the bioavailability of FA .