论文部分内容阅读
[目的]研究锆对小鼠肾脏的损伤。[方法]将50只健康6~8周龄清洁级雄性昆明小鼠按体质量随机分成5组,分别为对照(生理盐水)组和0.855、1.710、3.420、17.100 mg/kg氧氯化锆染毒组,每组10只。采用腹腔注射方式进行染毒,染毒剂量为20 mL/kg,每日1次,连续染毒30 d。测定血清肌酐(Scr)和尿素氮(BUN)的含量及肾脏匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性和丙二醛(MDA)的含量。[结果]与对照组相比,仅17.100 mg/kg氧氯化锆染毒组小鼠血清中Scr含量明显高于对照组,差异有统计学意义(P<0.05)。随着染毒剂量的升高,小鼠血清中Scr的含量呈上升趋势(r=0.609,P<0.001)。此外,与对照组比较,1.710、3.420、17.100 mg/kg氧氯化锆染毒组小鼠肾脏匀浆中GSH-Px、SOD活性均下降,17.100 mg/kg氧氯化锆染毒组小鼠肾脏匀浆中MDA的含量升高,差异有统计学意义(P<0.05)。随着氧氯化锆染毒剂量的升高,小鼠肾脏匀浆中的MDA含量呈上升趋势(r=0.755,P<0.001),SOD、GSH-Px活性均呈下降趋势(r=-0.570,P<0.001;r=-0.536,P=0.001)。0.855、1.710、3.420 mg/kg氧氯化锆染毒组小鼠肾脏肾小管上皮细胞轻度肿胀,有轻微炎细胞浸润,其中3.420 mg/kg染毒组小鼠肾小管上皮细胞部分损伤脱落;17.100 mg/kg染毒组小鼠肾脏肾小管管腔狭窄,多数上皮细胞损伤脱落。[结论]在本实验范围内,低剂量的氧氯化锆对小鼠肾脏有轻微损伤,而高剂量(>17.100 mg/kg)的氧氯化锆对小鼠肾脏有明显的损伤作用。
[Objective] The research aimed to study the damage of zirconium on mouse kidney. [Methods] Fifty healthy male Kunming mice, aged 6-8 weeks, were randomly divided into five groups according to their body weight, which were control (saline) group and 0.855,1.710,3.420,17.100 mg / kg zirconium oxychloride dyed Toxic group, 10 in each group. The rats were injected intraperitoneally with the dose of 20 mL / kg once daily for 30 days. The contents of serum creatinine (Scr) and blood urea nitrogen (BUN) and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) content. [Results] Compared with the control group, the Scr content in the serum of mice treated with only zirconium oxychloride at 17.100 mg / kg was significantly higher than that of the control group, with statistical significance (P <0.05). With the increase of exposure dose, the content of Scr in mouse serum increased (r = 0.609, P <0.001). In addition, compared with the control group, the GSH-Px and SOD activities in renal homogenates of mice exposed to 1.7,0.3.420 and 17.100 mg / kg of oxychloride decreased, and the activity of GSH-Px and SOD decreased The content of MDA in renal homogenate increased, the difference was statistically significant (P <0.05). With the increase of the dose of oxychloride injection, the content of MDA in kidney homogenates increased (r = 0.755, P <0.001) and the activities of SOD and GSH-Px decreased (r = -0.570 , P <0.001; r = -0.536, P = 0.001). 0.855, 1.710 and 3.420 mg / kg, respectively. The renal tubular epithelial cells in mice exposed to zirconium oxychloride slightly swollen with slight infiltration of inflammatory cells, and the renal tubular epithelial cells in 3.420 mg / Kidney renal tubular stenosis was found in the 17.100 mg / kg group, most epithelial cells were damaged. [Conclusion] In this experiment, low doses of zirconium oxychloride slightly damaged the kidneys of mice, whereas high doses (> 17.100 mg / kg) of zirconium oxychloride had obvious damage to the kidneys of mice.