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以重要的热带花卉蜻蜓凤梨(Aechemia fasciata)为材料,基于转录组数据,利用c DNA末端快速扩增(rapid amplification of c DNA ends,RACE)技术克隆了APETALA2/Ethylene Response Element Binding Protein(AP2/EREBP)家族的一个基因,并将其命名为Af DREB2C。Af DREB2C c DNA全长848 bp,有一个594 bp的开放阅读框(open reading frame,ORF),编码197个氨基酸残基。Af DREB2C蛋白分子量为21.498 8 k D,理论等电点为4.65,是一类酸性亲水蛋白,且预测定位于细胞核。实时荧光定量PCR(q RT-PCR)结果表明,Af DREB2C在不同株龄的蜻蜓凤梨不同组织中均有表达,但在很多生殖器官中的表达量很低。此外,Af DREB2C响应了外源乙烯处理,但在成株的不同组织中响应模式不尽相同。本研究为解析Af DREB2C在蜻蜓凤梨生长发育中的机制、为通过基因工程手段调控凤梨科植物生长发育奠定了理论基础。
Based on the transcriptome data, APETALA2 / Ethylene Response Element Binding Protein (AP2 / EREBP) was cloned by rapid amplification of c DNA ends (RACE) using the Aegicera fasciata, an important tropical flower. ) Family of genes, and named Af DREB2C. Af DREB2C c DNA is 848 bp in length and has a 594 bp open reading frame (ORF) encoding 197 amino acid residues. The molecular weight of Af DREB2C protein is 21.498 8 kD and its theoretical isoelectric point is 4.65. It is a kind of acidic hydrophilic protein and is predicted to be located in the nucleus. Real-time quantitative PCR (q RT-PCR) results showed that Af DREB2C expression in different tissues of different age groups of dragonfly pineapple, but in many reproductive organs expression is low. In addition, Af DREB2C responds to exogenous ethylene treatment, but responses vary among different adult tissues. This study is to analyze the mechanism of Af DREB2C in the growth and development of the dragonfly pineapple and lay a theoretical foundation for the regulation of growth and development of the bromeliads plant by means of genetic engineering.