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采用双抗体夹心ＥＬＩＳＡ法对６６例乙型肝炎和２０例健康献血员血清ＴＮＦ－α、ＩＦＮ－γ、ＩＬ－６和ＩＬ－８进行检测。结果显示：各临床型乙型肝炎血清ＴＮＦ－α、ＩＬ－６水平均明显高于健康献血员（Ｐ＜０．０５），除急性肝炎外，其它型肝炎血清ＩＦＮ－γ、ＩＬ－８水平亦明显高于健康对照组（Ｐ＜０．０５）；重型肝炎组升高更为明显，与急性肝炎组比较，ＴＮＦ－α、ＩＦＮ－γ、ＩＬ－６、ＩＬ－８水平前者明显高于后者，差异显著（Ｐ＜０．０５）；各型乙型肝炎患者ＴＮＦ－α与ＩＦＮ－γ、ＩＬ－６、ＩＬ－８呈正相关，相关系数ｒＩＦＮ＝０．２４，Ｐ＜０．０５，ｒＩＬ－６＝０．３５、Ｐ＜０．０５，ｒＩＬ－８＝０．４４、（Ｐ＜０．０５）；ＴＮＦ－α、ＩＦＮ－γ、ＩＬ－６、ＩＬ－８与血清总胆红素（ＴＢｉＬ）呈正相关（Ｐ＜０．０５）；动态观察急性肝炎和重型肝炎病程中诸因子的变化，发现ＩＦＮ－γ在病程初和肝昏迷开始时水平最高，而ＴＮＦ－α、ＩＬ－６、ＩＬ－８随病情加重而逐渐上升，肝损伤最重时达高峰，随病情好转逐渐下降。结果提示：细胞因子异常增高与肝脏炎症有关，可能是通过各种途径共同导致肝细胞损伤。
The serum levels of TNF-α, IFN-γ, IL-6 and IL-8 in 66 hepatitis B patients and 20 healthy blood donors were detected by double antibody sandwich ELISA. The results showed that the serum levels of TNF-α and IL-6 in all clinical hepatitis B were significantly higher than those in healthy donors (P <0.05). Except for acute hepatitis, the levels of serum IFN-γ and IL-8 (P <0.05). The levels of TNF-α, IFN-γ, IL-6 and IL-8 in the severe hepatitis group were significantly higher than those in the acute hepatitis group (P <0.05). There was a positive correlation between TNF-α and IFN-γ, IL-6 and IL-8 in patients with various types of hepatitis B, the correlation coefficient rIFN was 0.24, P <0.05 (RIL-6 = 0.35, P <0.05, rIL-8 = 0.44, P <0.05); TNF-α, IFN-γ, IL-6 and IL- (TBiL) was positively correlated (P <0.05). Dynamic changes in the pathogenesis of acute hepatitis and severe hepatitis were observed. It was found that IFN-γin the early course of disease and hepatic coma The highest level, and TNF-α, IL-6, IL-8 with the condition gradually rises increased, and reached a peak when the most severe liver injury, with the condition improved gradually decline. The results suggest that: the abnormal increase of cytokines and liver inflammation may be caused by a variety of ways together lead to liver cell damage.