Investigation of the Lipophilicity of 2-Benzoylpyridine-Thiosemicarbazone Based on the Ion Transfer

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The ion transfer reaction of 2-benzoylpyridine-thiosemicarbazone (HL), which has antimicrobial and antifungal properties and anticancer activity, has been studied to determine its lipophilicity by cyclic voltammetry at the water/1,2-dichloroethane (1,2-DCE) interface. The physicochemical parameters such as standard partition coefficient (lgPI)andthestandardGibbsenergyoftransfer(“G0tr,,wI ”o)oftheprotonatedformoftheligandwere measured asa function of pH in aqueous phase. The protonated form of the ligand exhibited reversible or quasi-reversible voltammograms at the1,2-DCEintherangeofpH1-5.Theprotonationconstantsoftheligand,pKa1andpKa2,weredeterminedspectrophotometrically and were found to be 12.14 and 3.24, respectively. The standard Gibbs energy of transfer (“G0tr,,wN” o) and the partition coefficient of neutral species (lgPN) were also determined by the shake-flask method. The standard Gibbs energy of transfer of this compound across the water/1,2-DCE interface was evaluated as the quantitative measure of its lipophilicity. The difference between lgPI and lgPN was related to the degree of charge delocalization and was used to evaluate qualitatively the lipophilicity of the ligand. The ion transfer reaction of 2-benzoylpyridine-thiosemicarbazone (HL), which has antimicrobial and antifungal properties and anticancer activity, has been studied to determine its lipophilicity by cyclic voltammetry at the water / 1,2-dichloroethane (1,2-DCE) interface. The physicochemical parameters such as standard partition coefficient (lgPI) and thestandardGibbsenergyoftransfer ( “G0tr ,, wI ” o) of the programmed condition ashelp and we measured as a function of pH in aqueous phase. The protonated form of the ligand demonstrated reversible or quasi-reversible voltammograms at the1 , 2-DCE variable range pHH1-5.Theprotonationconstantsoftheligand, pKa1andpKa2, weredeterminedspecometrically and were found to be 12.14 and 3.24, respectively. The standard Gibbs energy of transfer ( “G0tr ,, wN ” o) and the partition coefficient of neutral species (lgPN) were also determined by the shake-flask method. The standard Gibbs energy of transfer of this compound across the water / 1,2-DCE interface was evaluated as th e quantitative measure of its lipophilicity. The difference between lgPI and lgPN was related to the degree of charge delocalization and was used to evaluate qualitatively the lipophilicity of the ligand.
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