Na2S2O3在中性水溶液中还原KMnO4可制备得到暗棕色的可溶性MnO2溶胶.所制备的MnO2溶胶透明、稳定,最大吸收波长位于357 nm处,平均粒径约40 nm.研究发现,所制备的MnO2纳米溶胶在酸性介质中与甲醛反应可产生弱的化学发光.考察了近30种药物分子在MnO2纳米溶胶-甲醛体系中的化学发光行为.结果表明,吩噻嗪类药物、氨基硫醇类药物等对该体系的化学发光信号具有显著的增强作用.据此,建立了利用这一化学发光体系测定五种吩噻嗪类药物和四种氨基硫醇类药物新的流动注射化学发光分析方法.所建立的方法被成功地用于片剂中奋乃静含量和猪饲料中盐酸氯丙嗪含量的测定.通过对化学发光光谱、荧光光谱、紫外可见吸收光谱等实验的研究,提出了可能的化学发光反应机理.所有反应的化学发光光谱的最大发射波长均位于630～640 nm间,与分析物的种类无关.当向反应体系中加入单线态氧清除剂叠氮化钠和三亚乙基二胺时,反应的化学发光强度被不同程度的抑制,这表明单线态氧可能是该化学发光反应的发光体. Na2S2O3 in neutral aqueous solution can be prepared KMnO4 dark brown soluble MnO2 sol prepared MnO2 sol transparent and stable, the maximum absorption wavelength at 357 nm, the average particle size of about 40 nm found that the prepared MnO2 nano The reaction of sol with formaldehyde in acidic medium can produce weak chemiluminescence.Chemical luminescence behavior of nearly 30 kinds of drug molecules in MnO2 nano-sol-formaldehyde system was investigated.The results showed that phenothiazines, aminothiols and the like The chemiluminescence signal of the system has a significant enhancement.Accordingly, a new flow injection chemiluminescence assay for the determination of five phenothiazines and four aminothiols using this chemiluminescence system was established The established method was successfully applied to the determination of perphenazine content in the tablets and the content of chlorpromazine hydrochloride in the pig feed.According to the experimental study of chemiluminescence, fluorescence and UV-Vis spectra, the possible chemical Luminescence reaction mechanism.The maximum emission wavelength of the chemiluminescence spectra of all the reactions are located at 630 ~ 640 nm, has nothing to do with the type of analyte.When to the reaction system When the singlet oxygen scavengers sodium azide and triethylenediamine are added, the chemiluminescence intensity of the reaction is suppressed to some extent, which indicates that singlet oxygen may be the emitter of the chemiluminescence reaction.