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为探讨水-水循环对生姜叶片的光保护作用,通过对生姜采取叶面喷施10 mmol·L-1碘乙酰胺(IA)与遮光50%交互处理,研究了自然强光+清水(T1)、遮光50%+清水(T2)、自然强光+10 mmol·L-1IA(T3)、遮光50%+10 mmol·L-1IA(T4)对生姜叶片叶绿素荧光参数、Mehler反应及超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)活性的影响.结果表明:处理过程中,T3、T4处理生姜叶片光合速率及最大光化学效率均持续降低,至处理9 d时,光合速率分别较处理前降低了64%和33.2%,最大光化学效率分别降低了16.5%和10.9%,Mehler反应分别升高了139.4%和72.6%,且SOD和APX活性显著增强,而T1、T2则无显著变化.处理6 d时,一天内各处理生姜叶片光合速率、最大光化学效率在午间均显著降低,叶片的Mehler反应及SOD、APX活性在午间均显著增强,但以T3变幅较大,T4次之,T1、T2较小.可见,外源IA抑制生姜叶片光合速率后,Mehler反应及活性氧清除酶活性增强,表明水-水循环在耗散生姜叶片过剩光能中起重要作用.
In order to investigate the photoprotective effect of water-water circulation on ginger leaves, natural glare + water (T1) was applied to treat ginger by spraying 10 mmol·L-1 iodoacetamide (IA) and shading 50% , Chlorophyll fluorescence parameters, Mehler reaction and superoxide dismutase (SOD) of ginger leaves under the light intensity of 50% + water (T2), natural light +10 mmol·L-1IA (T3) and shading of 50% +10 mmol·L- The activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in leaves of Ginger were studied.The results showed that the photosynthetic rate and maximum photochemical efficiency of Ginger treated with T3 and T4 decreased continuously, and the photosynthetic rate The maximum photochemical efficiency decreased by 16.5% and 10.9%, respectively, and the Mehler reaction increased by 139.4% and 72.6% respectively, and the activities of SOD and APX increased significantly, while those of T1 and T2 were not significant The maximum photochemical efficiency decreased significantly at midday, the Mehler reaction and the activities of SOD and APX in leaves increased significantly at midday, but the amplitude of T3 was larger and T4 , T1, T2 is small.It can be seen that the exogenous IA inhibited the photosynthetic rate of ginger leaves, Mehler reaction and activity Oxygen scavenging enzyme activity is enhanced, indicating that water-water circulation plays an important role in dissipating excess light energy in ginger leaves.