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目的探讨苏州地区女性宫颈细胞中23种人乳头瘤病毒(HPV)感染的基因型分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对1827例女性宫颈细胞标本进行23种HPV基因型别的检测,并对其受检者进行相关资料分析。结果 1827例中检出HPV感染者611例,总的HPV感染率为33.44%,其中1种型别感染的阳性检出率为22.44%;1种型别感染中HPV16型为114例,其阳性检出率为6.24%,是最主要的感染型别;其次HPV58型为48例,其阳性检出率为2.63%;多重HPV感染201例,其阳性检出率为11.00%;其中多重HPV16型68例,占多重感染的33.83%,是多重感染的主要型别,其次是多重HPV11型32例,占多重感染的15.92%。结论 HPV16型、58型1种型别及16型和11型多重型别是感染苏州地区女性宫颈细胞感染的主要基因型别,基因扩增结合基因芯片技术一次可检测23种HPV基因型别,特异性强,敏感性高,可应用于宫颈细胞标本HPV感染的检测。
Objective To investigate the genotype distribution and clinical significance of 23 human papillomavirus (HPV) infection in female cervical cells in Suzhou area. Methods Genomic DNA microarray was used to detect 23 HPV genotypes in 1827 cases of female cervical cells. The related data were analyzed. Results Among the 1827 cases, 611 cases were detected with HPV infection, the total HPV infection rate was 33.44%. The positive detection rate of one type infection was 22.44%. One type of infection was HPV16, 114 cases were positive The detection rate was 6.24%, which was the most important type of infection. Second, the HPV58 type was 48 cases, the positive rate was 2.63%. The multiple HPV infection was 201 cases, the positive rate was 11.00% 68 cases, accounting for 33.83% of multiple infections, is the main type of multiple infections, followed by multiple HPV11 type in 32 cases, accounting for 15.92% of multiple infections. Conclusion HPV16 type, 58 type and multiple genotypes 16 and 11 are the main genotypes of cervical cytotoxicity in Suzhou women. Twenty-three HPV genotypes can be detected by gene amplification combined with gene chip technology. Specificity, high sensitivity, can be applied to the detection of HPV infection in cervical cell specimens.