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行全耳蜗人工外淋巴灌注,观察不同灌注速度对蜗内电位的影响,并作耳蜗连续切片,观察内耳结构的形态变化。灌注速度分别为:5μl/min,50μl/min,100μl/min,250μl/min,500μl/min。当灌注速度为100μl/min时可使蜗内电位下降,但停灌后电位可恢复。而灌注速度进一步提高为250μl/min以上后,蜗内电位即出现不可逆的下降。在灌注速度分别为5μl/min,50μl/min和100μl/min时,连续切片观察未发现异常。在250μl/min组中有1耳发现有前庭膜塌陷。在500μl/min组中发现3耳有前庭膜塌陷或破裂。人工外淋巴灌注速度不宜过快,在豚鼠耳所作试验提示,流速以不超过100μl/min为宜。
The cochlear artificial peripheral lymphatic perfusion was performed to observe the effects of different perfusion rates on intracochlear potentials. Serial cochlear sections were made to observe the morphological changes of inner ear structures. Perfusion rates were: 5 μl / min, 50 μl / min, 100 μl / min, 250 μl / min, 500 μl / min. When the perfusion rate of 100μl / min can make intracochlear potential decreased, but after stopping the potential recovery. However, when the perfusion rate is further increased to 250 μl / min or more, the intracochlear potential is irreversibly decreased. At perfusion rates of 5 μl / min, 50 μl / min and 100 μl / min, no abnormalities were observed in serial sections. Vestibular collapse was found in 1 ear in 250 μl / min group. Vestibular collapse or rupture was found in 3 ears in 500 μl / min group. Artificial lymphatic perfusion rate should not be too fast, the test made in guinea pig ears suggest that the flow rate to not more than 100μl / min is appropriate.