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目的：研究金雀异黄素对猪血小板聚集和［Ｃａ２＋］ｉ的影响．方法：用比浊法检测血小板聚集和用Ｆｕｒａ２荧光法检测［Ｃａ２＋］ｉ．结果：金雀异黄素强烈地抑制凝血酶（２５０Ｕ·Ｌ－１）诱导的猪血小板聚集，当金雀异黄素的浓度为５和２０μｍｏｌ·Ｌ－１时，它对聚集的抑制率分别为５２％和７３％．当血小板胞外存在Ｃａ２＋１ｍｍｏｌ·Ｌ－１时，金雀异黄素抑制凝血酶（５００Ｕ·Ｌ－１）诱导的血小板［Ｃａ２＋］ｉ的升高；金雀异黄素对凝血酶诱导的钙释放无影响．结论：金雀异黄素是潜在的抗血小板药物，抑制钙内流导致抑制［Ｃａ２＋］ｉ的升高，这与其抑制血小板聚集有关．
Objective: To investigate the effects of genistein on platelet aggregation and [Ca2 +] i in pigs. Methods: Platelet aggregation was detected by turbidimetry and [Ca2 +] i was detected by Fura2 fluorescence assay. Results: Genistein strongly inhibited platelet aggregation induced by thrombin (250U · L-1). When genistein concentration was 5 and 20 μmol·L-1, For 52% and 73%. Genistein inhibited the increase of platelet [Ca2 +] i induced by thrombin (500 U · L-1) when Ca2 + 1 mmol·L-1 was present extracellularly in platelets; the effect of genistein on thrombin-induced calcium release no effect. CONCLUSION: Genistein is a potential antiplatelet agent. Inhibition of calcium influx leads to inhibition of [Ca2 +] i elevation, which is related to its inhibition of platelet aggregation.