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目的:观察大蒜新素对不同刺激剂所致分离大鼠脑细胞内游离钙的影响。方法:以Fura 2-AM为细胞内游离钙的荧光指示剂,用AR-CM-MIC阳离子测定系统,直接测定了分离新生大鼠脑细胞内游离钙([Ca~(2+)]_i)值,观察了大蒜新素的影响。结果:大蒜新素对脑细胞静息[Ca~(2+)]_i无明显影响,大蒜新素1-100μmol·L~(-1)能剂量依赖性地抑制高K~+和谷氨酸引起的[Ca~(2+)]_i升高,其中IC_(50)分别为59.7和69.9μmol·L~(-1),高剂量大蒜新素100μmol·L~(-1)能抑制去甲肾上腺素引起的[Ca~(2+)]_i升高。结论:大蒜新素对高K~+、去甲肾上腺素及谷氨酸引起的[Ca~(2+)]_i升高的抑制作用可能是其抗脑缺血作用机制之一。
OBJECTIVE: To observe the effect of allitridin on intracellular free calcium in isolated rat brain cells induced by different stimulants. METHODS: Fara 2-AM was used as a fluorescent indicator of intracellular free calcium. The AR-CM-MIC cation measurement system was used to directly measure the intracellular free calcium in isolated neonatal rats ([Ca 2+ ]]_i). Values were observed for the effect of alliin. RESULTS: Garlicin had no significant effect on the resting [Ca 2+ ]_i of brain cells. Allitridi 1-100 μmol·L -1 dose-dependently inhibited the high K 2+ and glutamate levels. The increase of [Ca 2+ ]_i was induced, IC 50 was 59.7 and 69.9 μmol·L -1 , respectively. High-dose garcinoxin 100 μmol·L -1 inhibited the elimination of Epinephrine-induced elevation of [Ca 2+ ]_i. Conclusion: The inhibitory effect of allitridin on the increase of [Ca 2+ ]_i induced by high K+, norepinephrine and glutamate may be one of the mechanisms of anti-cerebral ischemia.