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采用ＭＴＴ比色法检测细胞增殖情况，用放免法（ＲＩＡ）测定胰岛素、胰高血糖素和胃泌素含量，氨基己糖含量用Ｎｅｕｈａｕｓ法测定，以期观察五肽胃泌素和生长抑素对体外培养的人胃癌细胞ＨＧＣ８０３和ＨＧＣ８２３生长的调节作用。结果显示，胃泌素对两株胃癌细胞增殖均有促进作用，以１×１０－５ｍｏｌ／Ｌ浓度最为明显，并使胃癌细胞氨基己糖含量明显增加（７．５８±０．６６ｖｓ４．２±０．３９ｐｇ／细胞，Ｐ＜０．０５））。生长抑素对两株胃癌细胞增殖均有抑制作用。以１×１０－６ｍｏｌ／Ｌ浓度最为明显，同时还抑制胃癌细胞分泌胃泌素和胰高血糖素的能力，并使胃癌细胞氨基己糖含量明显减少（２．６２±０．２９ｖｓ４．２±０．３９ｐｇ／细胞，Ｐ＜０．０５）。胃肠激素可调节胃癌细胞的生长，但尚有许多问题待阐明。
MTT colorimetric assay was used to measure cell proliferation. Insulin, glucagon, and gastrin content were determined by radioimmunoassay (RIA). The content of hexosamine was measured by Neuhaus method to observe the effect of pentagastrin and somatostatin. Regulation of growth of human gastric cancer cells HGC803 and HGC823 in vitro. The results showed that gastrin promoted the proliferation of the two gastric cancer cells, with the concentration of 1×10-5 mol/L being the most obvious, and significantly increasing the content of hexosamine in gastric cancer cells (7.58±0.66 vs 4.2%). 0.39 pg/cell, P<0.05)). Somatostatin inhibited the proliferation of both gastric cancer cells. The most obvious concentration was 1×10-6 mol/L. At the same time, gastric cancer cells were also inhibited in the secretion of gastrin and glucagon, and the content of hexosamine in gastric cancer cells was significantly reduced (2.62±0.29 vs. 4.2±). 0.39 pg/cell, P<0.05). Gastrointestinal hormone can regulate the growth of gastric cancer cells, but there are still many problems to be elucidated.