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尽管棉花被认为是耐盐植物.且不同的品种间已观察到耐盐性的变异,但是,棉花耐盐性的传递机制仍然没有解决.本研究的目的是探讨耐盐品种是否比盐敏感品种含有基本(或诱发产生)较高水平的抗氧化剂.在温室内,用0和150mMNaCl处理耐盐棉花品种(Acala1517—88和Acala1517—SR2)和盐敏感品种(Stoneville825和Deltapine50)的植株,测定它们生长状况和抗氧化剂容量的差异,结果表明,150mMNaCl处理,使Delatpine50和Stoneville825品种植株的生长对比照下降40%以上,而Acala品种植株的生长仅下降不到30%.耐盐品种Acala的过氧化氨酶(121~215%)和α-维生素E(312~340%)的基本水平较高.盐处理后,耐盐品种过氧化物酶活性提高了38~72%,谷胱甘肽还原酶活性提高了55~101%;而盐敏感品种这些酶活性则保持稳定或下降.生长在150mMNaCl条件下,耐盐品种比盐敏感品种显示出氧化抗坏血酸和还原抗坏血的比率低,还原谷胱甘肽和氧化谷胱甘肽的比率高.在一次盐胁迫处理后,Deltapine50的脂质过氧化水平比Acala1517—88高51%?
Although cotton is considered a salt-tolerant plant. Salt tolerance variation has been observed among different varieties. However, the mechanism of salt tolerance in cotton remains unsolved. The purpose of this study was to investigate whether salt-tolerant varieties contain higher (or induced) higher levels of antioxidants than salt-sensitive varieties. Plants from salt-tolerant cotton varieties (Acala 1517-88 and Acala 1517-SR2) and salt-sensitive varieties (Stoneville 825 and Deltapine 50) were treated with 0 and 150 mM NaCl in a greenhouse and the differences in their growth status and antioxidant capacity were measured and the results showed that 150 mM NaCl , The growth of Delatpine 50 and Stoneville 825 plants was reduced by more than 40% compared to the control, while the growth of Acala plants was only reduced by less than 30%. The salt tolerance variety Acala has a higher basic level of peroxidase (121-215%) and alpha-tocopherol (312-340%). After salt treatment, the peroxidase activity of salt-tolerant cultivars increased by 38-72% and the glutathione reductase activity by 55-101%, while those of salt-sensitive cultivars remained stable or decreased. Growing at 150 mM NaCl, salt-tolerant cultivars showed lower rates of oxidized ascorbic acid and reduced ascorbate than salt-sensitive cultivars, and a high rate of reduction of glutathione and oxidized glutathione. After a salt stress treatment, Deltapine50 lipid peroxidation level than Acala1517-88 high 51%?