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目的通过测定子痫前期患者母血及新生儿脐血中血小板活化因子乙酰水解酶(platelet-activating factor-acetylhydrolase,PAF-AH)水平,探讨其在子痫前期中的作用。方法选取妊娠晚期子痫前期患者46例作为子痫前期组(其中轻度子痫前期21例,重度子痫前期25例),30例正常晚期孕妇作为对照组;采用酶联免疫法测定两组母血及脐血中PAF-AH活性,分析其对子痫前期发病的影响。结果 1子痫前期组PAF-AH活性[(10.52±3.11)μmol·min-1·L-1]与对照组[(11.40±2.88)μmol·min-1·L-1]比较,差异无统计学意义(P>0.05);但重度子痫前期组PAF-AH活性[(8.23±1.41)μmol·min-1·L-1]与对照组比较,差异有统计学意义(P<0.05),重度子痫前期组PAF-AH活性与轻度组[(12.81±2.60)μmol·min-1·L-1]比较,差异有统计学意义(P<0.001);2子痫前期组脐血PAF-AH活性[(6.09±2.10)μmol·min-1·L-1]与对照组[(5.75±1.96)μmol·min-1·L-1]比较,差异无统计学意义(P>0.05);轻度子痫前期组脐血PAF-AH活性[(6.58±2.68)μmol·min-1·L-1]与重度组[(5.69±1.50)μmol·min-1·L-1]和对照组比较,差异均无统计学意义(P>0.05);3 PAF-AH活性与子痫前期程度呈负相关(r=-0.405,P<0.05)。结论重度子痫前期患者中PAF-AH活性降低,其活性与子痫前期病情程度呈负相关,可能在子痫前期病情的发生发展中起着重要作用。
Objective To investigate the role of platelet-activating factor-acetylhydrolase (PAF-AH) in maternal blood of preeclampsia and newborn umbilical cord blood. Methods Forty-six pregnant women with preeclampsia were enrolled in this study. The preeclampsia group (21 mild preeclampsia and 25 severe preeclampsia) and 30 normal late pregnant women were selected as the control group. Enzyme-linked immunosorbent assay PAF-AH activity in maternal blood and umbilical cord blood, and analyze its impact on the incidence of preeclampsia. Results 1 Compared with the control group [(11.40 ± 2.88) μmol · min-1 · L-1], PAF-AH activity in preeclampsia group [(10.52 ± 3.11) μmol · min-1 · L-1] (P> 0.05). However, the PAF-AH activity in severe preeclampsia group was significantly higher than that in control group [(8.23 ± 1.41) μmol · min-1 · L-1] PAF-AH activity in severe preeclampsia group was significantly higher than that in mild group [(12.81 ± 2.60) μmol · min-1 · L-1] (P <0.001); 2 PAF-AH activity in preeclampsia group (6.09 ± 2.10) μmol · min-1 · L-1] had no significant difference with the control group [(5.75 ± 1.96) μmol · min-1 · L-1] (6.58 ± 2.68) μmol · min-1 · L-1 in cord blood of mild preeclampsia group and 5.69 ± 1.50 μmol · min-1 · L-1 in severe group There was no significant difference between the two groups (P> 0.05). 3 PAF-AH activity was negatively correlated with the degree of preeclampsia (r = -0.405, P <0.05). Conclusions PAF-AH activity is decreased in patients with severe preeclampsia. The activity of PAF-AH is negatively correlated with the severity of preeclampsia and may play an important role in the pathogenesis of preeclampsia.