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目的评价重组人胸腺肽α_1(rh-Tα_1)对大鼠肝组织细胞色素P450(CYP450)基因表达的影响。方法SD大鼠经腹腔注射不同剂量的rh-Tα_1(150、300和600μg/kg),14 d后从肝组织中提取总RNA,应用逆转录-聚合酶链反应(RT-PCR)检测CYP3A1、CYP1A2和CYP2E1 mRNA的表达。结果与空白对照组相比,rh-Tα_1可显著诱导CYP3A1、CYP 1A2和CYP 2E1的基因表达。结论rh-Tα_1通过诱导CYP450酶系的mRNA表达影响其活性。
Objective To evaluate the effect of recombinant human thymosin α_1 (rh-Tα_1) on the expression of cytochrome P450 (CYP450) in rat liver. Methods SD rats were injected intraperitoneally with different dosages of rh-Tα_1 (150, 300 and 600 μg / kg), and total RNA was extracted from the liver tissue after 14 days. The CYP3A1 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) CYP1A2 and CYP2E1 mRNA expression. Results Compared with the blank control group, rh-Tα_1 significantly induced the gene expression of CYP3A1, CYP 1A2 and CYP 2E1. Conclusion rh-Tα_1 can affect its activity by inducing CYP450 mRNA expression.