利用茜草科植物毛金鸡纳(Cinchona pubescens)嫩枝中分离出的内生植物真菌炭角菌(Xylaria sp.),对金鸡纳生物碱进行了微生物转化。 毛金鸡纳嫩枝切成约1cm长,用自来水洗涤10min后,依次用70% 乙醇灭菌1min、5.3%次氯酸钠灭菌5min和75% 乙醇灭菌0.5min。把每一段再切成二段,放 The endophytic fungus Xylaria sp. isolated from the twigs of Cinchona pubescens was used to microbial transformation of cinchona alkaloids. The young shoots of S. philoxeroides were cut into approximately 1 cm long shoots, washed with tap water for 10 minutes, then sterilized with 70% ethanol for 1 minute, sterilized with 5.3% sodium hypochlorite for 5 minutes, and sterilized with 75% ethanol for 0.5 minutes. Cut each section into two sections and put