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随着甲、乙型肝炎特异性诊断方法的建立,证明大多数输血后肝炎并非由甲、乙肝病毒所致。由于长期未能分离出这种肝炎病毒,也未建立特异诊断方法,其诊断主要依靠排除甲、乙型肝炎及其他肝炎病毒感染,故长期称之为血源性非甲非乙肝炎。目前关于血源性非甲非乙肝炎已获得里程碑式进展。 1.已从血源性非甲非乙肝炎病毒基因组中分离出一cDNA克隆:美国Chiron公司研究者绕过分离病毒的传统研究方法,利用现代分子生物学技术,从建立、筛选病毒基因入手,首先在含有非甲非乙肝炎因子血浆中建立一个以随机引物合成的cDNA(互补性DNA)库,将其分别插入细菌基因中进行表达,然后用一
With the establishment of A and B specific diagnostic methods, it is proved that most post-transfusion hepatitis is not caused by A and B viruses. Hepatitis A virus has not been isolated for a long time and no specific diagnostic method has been established. Its diagnosis mainly depends on the exclusion of hepatitis A and hepatitis B virus infection, so it has long been called blood-based non-A non-B hepatitis. There is currently a milestone on blood-borne non-A non-B hepatitis. 1. A cDNA clone has been isolated from the blood-borne non-A, non-B hepatitis virus genome. The Chiron researchers in the United States bypassed the traditional research methods of virus isolation and used modern molecular biology techniques. Starting with the establishment and screening of viral genes, Firstly, a cDNA library (random amplified DNA) synthesized by random primers was established in the plasma containing non-A, non-B hepatitis, and inserted into bacterial genes for expression respectively,