The Least Squares Support Vector Machines (LS-SVM) is an improvement to the SVM.Combined the LS-SVM with the Multi-Resolution Analysis (MRA), this letter proposes the Multi-resolution LS-SVM (MLS-SVM). The proposed algorithm has the same theoretical framework as MRA but with better approximation ability. At a fixed scale MLS-SVM is a classical LS-SVM, but MLS-SVM can gradually approximate the target function at different scales. In experiments, the MLS-SVM is used for nonlinear system identification, and achieves better identification accuracy.