论文部分内容阅读
目的:对蒙古族药文冠木普通粉与超微粉中主要有效成分表儿茶素的体外溶出进行考察。方法:采用HPLC测定表儿茶素含量。色谱条件为Agilengt Eclipse-C18色谱柱(4.6 mm×250 mm,5μm),检测波长280 nm,流动相乙腈-0.04%磷酸水溶液(15∶85),流速1.0 m L·min-1,进样量20μL。测定文冠木普通粉与超微粉的溶出量和溶出速率,并对体外溶出行为进行溶出动力学分析。结果:普通粉与超微粉中表儿茶素的溶出量分别为5.868,8.440 mg·g-1,二者中表儿茶素的体外溶出曲线均符合Weibull分布。普通粉中表儿茶素的溶出参数T50,Td,T70,T80,T90分别为0.476,1.952,2.939,5.488,9.961 min,超微粉则依次为0.180,0.348,0.469,0.768,1.249 min。超微粉中表儿茶素的溶出速率明显高于普通粉(P<0.01),单位时间内(1 h)体外溶出量较普通粉提高约44%。结论:超微粉碎能明显促进文冠木中表儿茶素的体外溶出速率及溶出量,说明超微粉碎技术应用于蒙古族药文冠木是切实可行的。
OBJECTIVE: To investigate the in vitro dissolution of epicatechin, the main active ingredient in the Mongolian medical crown wood powder and superfine powder. Methods: The content of epicatechin was determined by HPLC. The chromatographic conditions were Agilengt Eclipse-C18 column (4.6 mm × 250 mm, 5 μm), detection wavelength 280 nm, mobile phase acetonitrile-0.04% phosphoric acid solution (15:85), flow rate 1.0 mL · min-1, 20 μL. The dissolution and dissolution rate of common and superfine powder were determined, and the dissolution kinetics was analyzed. Results: The dissolution rates of epicatechin in common powder and superfine powder were 5.868,8.440 mg · g-1, respectively. The in vitro dissolution curves of epicatechin were in accordance with Weibull distribution. The dissolution parameters of epicatechin in common powder, T50, Td, T70, T80 and T90 were 0.476, 1.952, 2.939, 5.488 and 9.961 min respectively, while those of superfine powder were 0.180,0.348,0.469,0.768,1.249 min. The dissolution rate of epicatechin in ultrafine powder was significantly higher than that of common powder (P <0.01). The dissolution rate of epicatechin in ultrafine powder was about 44% higher than that of ordinary powder in 1 h. CONCLUSION: Ultrafine pulverization can obviously promote the dissolution rate and dissolution rate of epicatechin in Wen Guan Mu. It is feasible to apply ultrafine pulverization technology to Mongolian medical crown culture.