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目的从分子水平检测胃粘膜中细胞因子ＩＬ－１βｍＲＮＡ的含量，以探讨幽门螺杆菌（Ｈｐ）感染对其影响。方法以竞争ＲＴ－ＰＣＲ方法检测１０例Ｈｐ感染者根除治疗前后胃粘膜中ＩＬ－１βｍＲＮＡ的表达量。结果根除治疗后粘膜中ＩＬ－１βｍＲＮＡ的表达量显著下降（Ｐ＜０．０１）。试验证明该方法简便、敏感、结果准确。结论Ｈｐ感染可诱导粘膜中产生过量的ＩＬ－１β
Objective To detect the content of cytokine IL-1βmRNA in gastric mucosa at molecular level to investigate the influence of Helicobacter pylori (Hp) infection on it. Methods The expression of IL-1βmRNA in gastric mucosa of 10 Hp infected patients before and after eradication therapy was detected by competitive RT-PCR. Results After eradication treatment, the expression of IL-1βmRNA in mucosa significantly decreased (P <0.01). The test proves that the method is simple, sensitive and accurate. Conclusion Hp infection can induce excessive IL-1β production in the mucosa