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Objective: FHL2 was previously identified to be a novel interacting factor of Id family proteins.The aim of this study was to investigate,the effects of FHL2 on Idl-mediated transcriptional regulation activity and its oncogenic activity in human breast cancer cells.Methods: Cell transfection was performed by Superfect reagent.Idl stably overexpressed MCF-7 cells was cloned by G418 screening.The protein level of JAI was detected by western blot analysis.Dual relative luciferase assays were used to measure the effect of E47-mediated transcriptional activity in MCF-7 human breast cancer cells.MTT assay was used to measure cell proliferation.Transwell assay was used to measure the invasive capacity of MCF-7 cancer cells.Results: The basic helix-loop-helix(bHLH) factor E47-mediated transcription activity was markedly repressed by Idl in MCF-7 cells.This Idl-mediated repression was effectively antagonized by FHL2 transduction.Overexpression of Idl markedly promoted the proliferation rate and invasive capacity of MCF-7 cells;however,these effects induced by Idl were significantly suppressed by overexpression of FHL2 in cells.Conclusion: FHL2 can inhibit the proliferation and invasiveness of human breast cancer cells by repressing the functional activity of Idl.These findings provide the basis for further investigating the functional roles of FHL2-Idl signaling in the carcinogenesis and development of human breast cancer.