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研究不同浓度螺旋藻激酶(spirulina kinase,SPK)对肾上腺素(Adr)损伤血管内皮细胞分泌组织型纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制剂(PAI-1)的影响。采用不同浓度SPK处理人脐静脉内皮细胞(HUVEC),MTT法检测不同浓度SPK对HUVEC活力影响;建立Adr损伤模型,酶联免疫吸附法检测细胞培养液中t-PA和PAI-1含量的变化。结果显示SPK浓度在10μg/mL~2 mg/mL范围内,细胞活力明显升高且不影响细胞分泌t-PA和PAI-1的量;SPK作用于Adr损伤模型,各剂量组均可降低Adr损伤下HUVEC分泌PAI-1的量,提高t-PA/PAI-1比值。本研究揭示了SPK可通过降低PAI-1、提高t-PA/PAI-1比值来实现保护Adr损伤的血管内皮细胞。
To investigate the effects of different concentrations of spirulina kinase (SPK) on the secretion of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) by epinephrine (Adr) Impact. Human umbilical vein endothelial cells (HUVECs) were treated with different concentrations of SPK. The effects of different concentrations of SPK on the activity of HUVECs were detected by MTT assay. The Adr injury model was established. The changes of t-PA and PAI-1 in cell culture medium were detected by enzyme-linked immunosorbent assay . The results showed that the SPK concentration in the range of 10μg / mL ~ 2mg / mL significantly increased cell viability and did not affect the amount of t-PA and PAI-1 secreted by cells; SPK treated Adr injury model, each dose group can reduce Adr The amount of PAI-1 secreted by HUVECs under injury increased the t-PA / PAI-1 ratio. This study revealed that SPK can protect Adr injured vascular endothelial cells by decreasing PAI-1 and increasing t-PA / PAI-1 ratio.